Identification of Yeast Strains Isolated from Agricultural Soils for Releasing Potassium-bearing Minerals

摘要

Thirty-five yeast strains were isolated from soil samples that were collected from different locations in Upper Egypt. The purified isolates were screened for the release potassium from mica on Aleksandrov agar medium. Two yeast isolates (KSY-29 and KSY-33) showed an ability to solubilize potassium by inducing clear zones around their colonies. They were identified as Pichia anomala and Rhodotorula glutinis, respectively, based on PCR analysis of the ITSI-26S region that was amplified by NL1/NL4 species-specific primers. The amount of K released from muscovite mica in the broth culture of the yeast isolates was measured after 5, 10, 15 and 20days of the incubation at 25 degrees C. Both yeast isolates were very effective in releasing K of muscovite in broth culture, recording 8.11-13.21g/ml that were released from muscovite mica after 20days of incubation. The inoculation of maize (Zea maize) plants with these yeast isolates under different K levels (25, 50 and 100 of recommended dose of potassium, RDK) as potassium sulfate was tested on growth and K uptake by these plants in the greenhouse. Significant increases (p < 0.05) in plant height, root and shoot dry weights as well as K uptakes by shoots and roots maize plants occurred through the inoculation with KSY-29 or KSY-33 isolates.