Critical assessment of fluorescence polarization measurements with a FACS IV cell sorter

摘要

The usefulness and limitations of the Bectonndash;Dickinson fluorescencehyphen;activated cell sorter FACS IV for fluorescence polarization measurements were examined. A set of tests to determine the characteristics of the detection geometry, the optical properties of the beam splitter, and the capability to process fluorescence polarization data is presented. Recommendations are provided for correcting instrumental deficiencies.