Isolation of Vindoline fromCatharanthus roseusby Supercritical Fluid Extraction

摘要

AbstractVindoline was extracted from the leaves ofCatharanthus roseusover the ranges of 35–70 °C and 100–300 bar using supercritical carbon dioxide with and without the addition of 3 wt ethanol as a cosolvent. The vindoline contents in the extracts were determined by HPLC and identified by LC/MS. The remarkable highest vindoline concentration, 58 wt , was obtained at the lowest temperature, 35 °C, and the highest pressure, 300 bar, of this study. The use of a cosolvent only slightly improved the extraction yields or selectivities at some experimental condi