Lipase and Esterase in Adipose Tissue and Liver

摘要

Rat epididymal adipose tissue contained lipase EC 3.1.1.3 and esterase EC 3.1.1.1which were separated by ammonium sulfate fractionation and gel filtration on a Sephadex G-200 column. On the other hand, rat liver contained a lipolytic enzyme, which seems to correspond to lipase, as judged from its behaviour on ammonium sulfate fractionation and gel filtration on Sephadex G-200, and from its substrate specificity.When the adipose tissue and liver lipase were treated with acetone, their behaviours on gel filtrations and substrate specificities were converted into those of the adipose tissue esterase