PRIMARY STUDY ON MECHANISM OF COLLAGEN SYNTHESIS INHIBITION IN CULTURED HUMAN FETAL HEPATOCYTES PRETREATED BY SALVIA MILTIORRHIZA BUNGE

摘要

The effects of salvia miltiorrhiza bunge (SMB) on collagen synthesis in the human fetal hepatocytes culture were studied. The collagen synthesis of hepatocytes were stimulated by the addition of carbon tetrachloride (CCl_4) to the culture medium, the concentration of type procollagen (PC) in the culture medium and the hydroxyproline (Hyp) in hepatocytes were determined, as well as the activity of se-dependent glutathione peroxidase (Se-GSH-Px) and the concentration of malondiadehyde (MDA) in the culture medium. A significant decrease in PC, Hyp and MDA production, and the significant increase in Se-GSH-Px activity were observed in the cultures pre-treated with 1 g L~(-1) SMB for 4 hours compared with the untreated cultures. Analysis of the Se-GSH-Px/MDA ratio in SMB pretreated group showed more marked increase compared to that of the untreated group (P<0. 01). There was a significant negative correlation between the ratio of Se-GSH-Px/MDA and the concentration of PC in SMB pretreated group (r= -0.9017, P <0.01). Our results indicate that SMB may suppress the collagen synthesis of cultured human fetal hepatocytes stimulated by CCl_4, and its mechanism may be related to the increase in Se-GSH-Px/MDA ratio and the enhancement of hepatocytes antioxidation capability.