Versatile Nickel(II) Scaffolds as Coordination-Induced Spin-State Switches for(19)F Magnetic Resonance-Based Detection

摘要

F-19 magnetic resonance (MR) based detection coupled with well-designed inorganic systems shows promise in biological investigations. Two proof-of-concept inorganic probes that exploit a novel mechanism for(19)F MR sensing based on converting from low-spin (S=0) to high-spin (S=1) Ni(2+)are reported. Activation of diamagneticNiL(1)andNiL(2)by light or beta-galactosidase, respectively, converts them into paramagneticNiL(0), which displays a single(19)F NMR peak shifted by >35 ppm with accelerated relaxation rates. This spin-state switch is effective for sensing light or enzyme expression in live cells using(19)F MR spectroscopy and imaging that differentiate signals based on chemical shift and relaxation times. This general inorganic scaffold has potential for developing agents that can sense analytes ranging from ions to enzymes, opening up diverse possibilities for(19)F MR based biosensing.