An Identification and a Quantitative Assessment of Phenolic Compounds in a Butanol Fraction of Extract from Cosmary Flowers (Tanacetum vulgare)

摘要

The costmary (Tanacetum vulgare L., syn.: Chrysanthemumvulgare (L). Bernh) is a perennial medicinal plant with a Euro-Asian type of a habitat. The costmary extracts have a wide spectrum of a biological and pharmacological activity. A drug with high choleretic and anti-inflammatory activity was created on the basis of the butanol fraction of the extract from the T. vulgare flowers in the All-Russian Institute of Medicinal and Aromatic Plants. The goal of this investigation was a determination of the qualitative composition and the quantitative content of phenolic compounds of the butanol fraction which are considered to be probably responsible for the biological activity of the extract. High performance liquid chromatography with diode-array detection in a combination with high-resolution mass spectrometry (UPLC-PDA-HRMS) was used for the analysis. We demonstrated that seven phenolic compounds were present in the butanol fraction of T. vulgare, including the first-isolated myricetin-3-glucoside. The content of the phenolic compounds achieved 90, and neochlorogenic, 3,5-, and 4,5-dicaffeoylquinic acids were the main compounds (their content was 69). Thus, the high content of caffeoylquinic acids could be responsible for the pharmacological activity of the drug based on the butanol fraction of the extract from the costmary flowers.