Synthesis and Glycogen Phosphorylase Inhibitory Activity of the Conjugates of 5-Chloroindoledicarboxylic Acid and Diazepine Derivatives

摘要

In this work, three novel amide derivatives with chloroindole as the core were synthesized through different experimental schemes. Subsequently, biological evaluation of these compounds as inhibitors of glycogen phosphorylase (GP) was described. The results showed that three compounds exhibited certain inhibitory activity against glycogen phosphorylase. Among which compound (Ic) showed the best inhibitory effect with the IC50 of 2.52 mu M. Finally, aiming at further understanding the interaction between the newly designed molecules and glycogen enbox1 phosphorylase, the possible binding mode were explored based on molecular docking simulations. The results showed that all target compounds could bind to glycogen phosphorylase, and all ligand molecules were docked within the active pocket.