首页> 外文期刊>Journal of Bioscience and Bioengineering >Co-culture of Bacillus amyloliquefaciens and recombinant Pichia pastoris for utilizing kitchen waste to produce fengycins
【24h】

Co-culture of Bacillus amyloliquefaciens and recombinant Pichia pastoris for utilizing kitchen waste to produce fengycins

机译:解淀粉芽孢杆菌与重组毕赤酵母共培养利用厨余垃圾生产枫霉素

获取原文
获取原文并翻译 | 示例
获取外文期刊封面目录资料

摘要

Kitchen waste (KW) is a vast potential source of fermentable substrates. To bio-convert the KW into high-value chemicals, we used KW as substrate for the production of fengycin by an artificial consortium containing Bacillus amyloliquefaciens HM618 producing fengycin and the engineering Pichia pastoris producing amylase, glucosidase, or lipases. The maximal amylase activity of the constructed amylase-producing engineering strain (recombinant P. pastoris GS115-amy98) reached 385.4 U.mL(-1). The engineering strain GS115-alpha-glu53 producing glucosidase reached an enzyme activity titer of 247.3 U.mL(-1), while the lipase activities of the engineering strains GS115-lip2, GS115-alpha-lip2, and GS115-lip7 were around 90.0 U.mL(-1), with no significant differences among them. Liquid chromatography-mass spectrometry (LC-MS) analysis showed that the components of fengycin synthesized by B. amyloliquefaciens HM618 were complex, including C14-C18 fengycins A, C13-C14 fengycins B, C16-C18 fengycins B, C16 fengycin B2 and some fengycin homologues with unsaturated fatty acid chains. The levels of fengycin were 15.9 mg.L-1 and 4.6 mg.L-1 under the co-culture with strain HM618 and the recombinant strains producing amylase and lipase, respectively. The maximal titer of fengycin was 21.2 mg.L-1 in the artificial consortia consisting of HM618 and the engineering strains producing glucosidase, amylase and lipase. Taken together, these results show that the co-culture of B. amyloliquefaciens HM618 and engineering strains producing amylase and lipase can promote the conversion of KW into fengycin. The work provides a new strategy for boosting the resource utilization of KW. (C) 2022, The Society for Biotechnology, Japan. All rights reserved.
机译:厨余垃圾 (KW) 是可发酵基质的巨大潜在来源。为了将KW生物转化为高价值的化学品,我们使用KW作为底物,通过含有产生风霉素的解淀粉芽孢杆菌HM618和产生淀粉酶、葡萄糖苷酶或脂肪酶的毕赤酵母的人工联合体生产凤凰素。构建的产淀粉酶工程菌株(重组帕斯托里斯 GS115-amy98)的最大淀粉酶活性达到 385.4 U.mL(-1)。工程菌株GS115-α-glu53产生葡萄糖苷酶的酶活性滴度为247.3 U.mL(-1),而工程菌株GS115-lip2、GS115-alpha-lip2和GS115-lip7的脂肪酶活性约为90.0 U.mL(-1),差异无统计学意义。液相色谱-质谱联用(LC-MS)分析表明,解淀粉芽孢杆菌HM618合成的风霉素成分复杂,包括C14-C18凤霉素A、C13-C14凤霉素B、C16-C18凤霉素B、C16凤霉素B2和一些具有不饱和脂肪酸链的凤霉素同系物。凤凰霉素水平为15.9mg。L-1和4.6mg。L-1 分别与菌株 HM618 和产生淀粉酶和脂肪酶的重组菌株共培养。风霉素的最大滴度为21.2mg。L-1 在由 HM618 和产生葡萄糖苷酶、淀粉酶和脂肪酶的工程菌株组成的人工联盟中。综上所述,这些结果表明,B.淀粉样脂肪体HM618和产生淀粉酶和脂肪酶的工程菌株可促进KW转化为风霉素。本研究为提高KW资源化利用率提供了新的策略。(C) 2022年,日本生物技术学会。保留所有权利。

著录项

获取原文

客服邮箱:kefu@zhangqiaokeyan.com

京公网安备:11010802029741号 ICP备案号:京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有
  • 客服微信

  • 服务号