Daily dynamic of transcripts abundance of Ma-LMM01-like cyanophages in two lowland European reservoirs

摘要

The light-dark cycle seems to influence the infection pattern of cyanophages. This may be related to their dependence on the photosynthetic process of their hosts -cyanobacteria. The present study examined the daily occurrence dynamic of Microcystis with the use of the 16S rRNA gene and the Ma-LMM01-like cyanophages with the use of the g91 gene in Jeziorsko and Sulejow reservoirs (Poland). Also, the daily expression pattern of mentioned cyanophages' gene was investigated. The daily dynamic of cyanophage was mainly associated with Microcystis occurrence. However, the g91 transcription exhibited greater activity during daylight and lowered within nighttime in both water bodies. The maximum transcription was found at 4:00 PM in Jeziorsko and 12:00 PM in Sulejow reservoirs. Additionally, the g91 gene fragments shared a high level of similarity with Microcystis specific cyanophages Ma-LMM01 and MaMV-DC strains. (c) 2021 European Regional Centre for Ecohydrology of the Polish Academy of Sciences. Published by Elsevier B.V. All rights reserved.