Recent results from single‐cell microspectrophotometry: Cone pigments in frog, fish, and monkey

摘要

AbstractA modified design of a previous microspectrophotometer (DMSP) is described that utilizes a phase‐modulated light beam. Applied to rod and cone outer segments, it can simultaneously measure optical density (OD) and linear dichroism (LD) spectra. LD spectra were found to be largely unaffected by the presence of light scattering and the absorption of the less anisotropic photoproducts. The bullfrog was found to have blue‐absorbing cones in addition to other previously known photoreceptors in amphibians. Four visual pigments of the sea raven are described. Preliminary data are presented on the four pigments in rhesus and cynomolgus monk