Lead halide perovskites with aggregation-induced emission feature coupled with gold nanoparticles for fluorescence detection of heparin

摘要

Herein, a new kind of lead halide perovskite (LHP, (C12H25NH3)(2)PbI4) with aggregation-induced emission (AIE) feature is developed as a fluorescent probe for heparin (Hep). The LHPs exhibit high emission when they aggregate in water. Interestingly, a few picomoles of dispersed gold nanoparticles (AuNPs) can quench the emission of LHPs, but the aggregated AuNPs are invalid. When protamine (Pro) is mixed with AuNPs at first, the negatively charged AuNPs aggregate through electrostatic interaction, producing the AIE recovery. Nevertheless, Hep disturbs the interaction between AuNPs and Pro due to its strong electrostatic interaction with Pro. Therefore, the dispersed AuNPs quench the fluorescence of LHPs again. A response linear range of Hep of 0.8-4.2 ng ml(-1) is obtained, and the detection limit is 0.29 ng ml(-1). Compared with other probes for determination of Hep with AuNPs, this strategy exhibits better sensitivity due to the small quantity of AuNPs used. Finally, it is also successfully applied to detect Hep in human serum samples with satisfactory recoveries.