Leaf wax n-alkane extraction: An optimised procedure

摘要

Highlights?An optimised procedure to extract leaf waxn-alkanes from leaves is proposed.?Accelerated solvent extraction performs better than ultrasound solvent extraction.?n-Hexane/dichloromethane 9:1 more effective than dichloromethane/methanol 9:1.?Extraction of intact leaves performs as well or better than shredded leaves.?No significant variation ofn-alkanes amounts/distribution alongC. mariscusleaves.AbstractLeaf waxn-alkanes are often extracted from modern plant material to describe their natural occurrence and understand the factors determining their potential as biomarkers for climate reconstruction. Despite several studies on the topic no standardised approach forn-alkan