pH-responsive and CD44-targeting by Fe3O4/MSNs-NH2 nanocarriers for Oxaliplatin loading and colon cancer treatment

摘要

In this work, we have introduced a promising method for performing colon HCT-116 cancer cells treatment by the usage of CD44 to function as the targeting receptors. For this purpose, we have synthesized superparamagnetic Fe3O4/Mesoporous silica nanoparticles (MSNs) through co-precipitation and sol-gel process and had the surface of the obtained nanocarriers (NCs) functionalized by NH2-bonding. The functionalized NCs have been characterized by the means of FT-IR spectroscopy, VSM, TEM, and FE-SEM. In the following, the values of drug loading and release have been measured through the application of ICP-OES analysis for 48 h, in which the drug release profile has been studied in both human blood (pH 7.4) and cancer cell (pH 5.0) conditions. According to the final results of MTT assay, the IC50 of Free drug and NCs -drug loaded has faced a decrease, from 7.5 mu g/mL to 3.2 mu g/mL, due to the fact that NH2 had been able to express a higher Oxaliplatin intracellular uptake and more CD44-binding than free Oxaliplatin. Therefore, this work has attempted to highlight these Fe3O4/MSNs-NH2 NCs potential in standing as a superior candidate for Oxaliplatin loading and colon cancer treatment.