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首页> 外文期刊>Biotechnology Progress >Cross‐linked enzyme aggregates of recombinant Candida antarctica lipase B for the efficient synthesis of olvanil, a nonpungent capsaicin analogue
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Cross‐linked enzyme aggregates of recombinant Candida antarctica lipase B for the efficient synthesis of olvanil, a nonpungent capsaicin analogue

机译：重组念珠菌抗原脂肪酶B的交联酶聚集体，用于高效合成Olvanil，一种非产辣椒素类似物

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Abstract > Despite the proven therapeutic role of capsaicin in human health, its usage is still hampered by its high pungency. In this sense, nonpungent capsaicin analogues as olvanil are a feasible alternative to the unpleasant sensations produced by capsaicin while maintaining a similar pharmacological profile. Olvanil can be obtained by a lipase‐catalyzed chemoenzymatic process. In the present work, recombinant Candida antarctica lipase B (CALB) was expressed in Pichia pastoris and subsequently immobilized by cross‐linked enzyme aggregate (CLEA) methodology for the synthesis of olvanil. The CALB‐CLEAs were obtained directly from the fermentation broth of P. pastoris without any purification step in order to assess the role of the contaminant proteins of the crude extract as co‐feeders. The CALB‐CLEAs were also bioimprinted to enhance the catalytic performance in olvanil synthesis. When CALB was precipitated with isopropanol, the obtained CALB‐CLEAs exhibited the highest activity in the synthesis of olvanil, regardless of the glutaraldehyde concentration. The maximum product synthesis was found at 72?hr obtaining 6.8 g?L ?1 of olvanil with a reaction yield of 16%. When CALB was bioimprinted with olvanil, the synthesis was enhanced 1.3 times, reaching 10.7 g?L ?1 of olvanil at 72?hr of reaction with a reaction yield of 25%. Scanning electron microscopy images indicated different morphologies of the CLEAs depending on the precipitating agent and the template used for bioimprinting. Recombinant CALB‐CLEAs obtained directly from the fermentation broth are a suitable alternative to commercial enzymatic preparations for the synthesis of olvanil in organic medium. </abstract> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> <div class="translation abstracttxt"> <span class="zhankaihshouqi fivelineshidden" id="abstract"> <span>机译：</span><Abstract Type =“Main”XML：Lang =“en”> <标题类型=“main”>抽象</ title> > 尽管辣椒素在人类健康中经过验证的治疗作用，但它的使用量仍然受到其高血症的阻碍。从这个意义上讲，作为奥凡尼的非产辣椒蛋白类似物是辣椒素产生的不愉快感觉的可行替代品，同时保持类似的药理学曲线。可以通过脂肪酶催化的化学酶方法获得奥替瓦里。在目前的工作中，重组 念珠菌南极洲</ i> 脂肪酶B（CALB）表达 Pichia Pastoris </ i> 随后通过交联酶聚集体（CLEA）方法来固定用于合成奥凡尔。直接从发酵液中获得CALB-CLEA p。 Pastoris </ i> 没有任何纯化步骤，以评估粗提物的污染物蛋白作为共送料器的作用。 CALB-CLEA也是BioIMIMPrinted以提高奥凡尼合成的催化性能。当CALB用异丙醇沉淀出来时，所获得的CALB-CLEA在合成奥凡尔的合成中表现出最高的活性，无论戊二醛浓度如何。最大产品合成在72℃下找到6.8g？l ？1 </ sup> 奥凡尔的反应产率为16％。当CALB与奥凡尔生物贴上时，合成增强了1.3倍，达到10.7克 ？1 </ sup> 在72℃的奥凡尼的反应产率为25％的反应。扫描电子显微镜图像根据沉淀剂和用于生物影响率的模板指示CLEA的不同形态。直接从发酵液中获得的重组CALB-CLE是用于合成有机培养基中Olvanil的商业酶促制剂的合适替代方法。 </ p> </摘要> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> </div> <div class="record"> <h2 class="all_title" id="enpatent33" >著录项</h2> <ul> <li> <span class="lefttit">来源</span> <div style="width: 86%;vertical-align: text-top;display: inline-block;"> <a href='/journal-foreign-15005/'>《Biotechnology Progress》</a> <b style="margin: 0 2px;">|</b><span>2019年第4期</span><b style="margin: 0 2px;">|</b><span>共11页</span> </div> </li> <li> <div class="author"> <span class="lefttit">作者</span> <p id="fAuthorthree" class="threelineshidden zhankaihshouqi"> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Diaz‐Vidal Tania&option=202" target="_blank" rel="nofollow">Diaz‐Vidal Tania;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Armenta‐Perez Vicente Paul&option=202" target="_blank" rel="nofollow">Armenta‐Perez Vicente Paul;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Rosales‐Rivera Luis Carlos&option=202" target="_blank" rel="nofollow">Rosales‐Rivera Luis Carlos;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Mateos‐Díaz Juan C.&option=202" target="_blank" rel="nofollow">Mateos‐Díaz Juan C.;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Rodríguez Jorge A.&option=202" target="_blank" rel="nofollow">Rodríguez Jorge A.;</a> </p> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zkzz" style="display: none;">展开▼</span> </div> </li> <li> <div style="display: flex;"> <span class="lefttit">作者单位</span> <div style="position: relative;margin-left: 3px;max-width: 639px;"> <div class="threelineshidden zhankaihshouqi" id="fOrgthree"> <p>Biotecnología Industrial Centro de Investigación y Asistencia en Tecnología y Dise?o del Estado de Jalisco CIATEJZapopan Jalisco Mexico;</p> <p>Biotecnología Industrial Centro de Investigación y Asistencia en Tecnología y Dise?o del Estado de Jalisco CIATEJZapopan Jalisco Mexico;</p> <p>Departamento de Ingeniería QuímicaUniversidad de GuadalajaraGuadalajara Jalisco Mexico;</p> <p>Biotecnología Industrial Centro de Investigación y Asistencia en Tecnología y Dise?o del Estado de Jalisco CIATEJZapopan Jalisco Mexico;</p> <p>Biotecnología Industrial Centro de Investigación y Asistencia en Tecnología y Dise?o del Estado de Jalisco CIATEJZapopan Jalisco Mexico;</p> </div> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zhdw" style="display: none;">展开▼</span> </div> </div> </li> <li > <span class="lefttit">收录信息</span> <span style="width: 86%;vertical-align: text-top;display: inline-block;"></span> </li> <li> <span class="lefttit">原文格式</span> <span>PDF</span> </li> <li> <span class="lefttit">正文语种</span> <span>eng</span> </li> <li> <span class="lefttit">中图分类</span> <span><a href="https://www.zhangqiaokeyan.com/clc/15.html" title="生物科学">生物科学;</a></span> </li> <li class="antistop"> <span class="lefttit">关键词</span> <p style="width: 86%;vertical-align: text-top;"> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=bioimprinting&option=203" rel="nofollow">bioimprinting;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=CALB&option=203" rel="nofollow">CALB;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=cross‐linked enzyme aggregates&option=203" rel="nofollow">cross‐linked enzyme aggregates;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=lipase&option=203" rel="nofollow">lipase;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=olvanil&option=203" rel="nofollow">olvanil;</a> </p> <div class="translation"> 机译：生物贴片;CALB;交联酶聚集体;脂肪酶;奥尔瓦尼尔; </div> </li> </ul> </div> </div> <div class="literature cardcommon"> <div class="similarity "> <h3 class="all_title" 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Picó&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Enrique A. Picó,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Carmen López&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Carmen López,</a> <span>-1</span> </span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：南极假丝酵母脂肪酶的磁性交联酶聚集体（mCLEAs）：一种高效稳定的生物柴油合成生物催化剂。</span> </p> </li> <li> <div> <b>11. </b><a class="enjiyixqcontent" href="/open-access_resources_thesis/010006694967.html ">Magnetic Cross-Linked Enzyme Aggregates (mCLEAs) of Candida antarctica lipase:An efficient and stable biocatalyst for biodiesel synthesis</a> <b>[O] </b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Cruz-Izquierdo, Álvaro&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Cruz-Izquierdo, Álvaro,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Picó, Enrique A.&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Picó, Enrique A.,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=López, Carmen&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">López, Carmen,</a> <span>2014</span> </span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：南极假丝酵母脂肪酶的磁性交联酶聚集体（mCLEAs）：一种高效，稳定的生物柴油合成生物催化剂</span> </p> </li> </ul> <ul style="display: none;"> <li> <div> <b>1. </b><a class="enjiyixqcontent" href="/patent-detail/06130422086415.html">CANDIDA ANTARCTICA-DERIVED NOVEL LIPASE VARIANT WITH ENHANCED ENZYME ACTIVITY</a> <b>[P]</b> . <span> 外国专利： <!-- 韩国专利： --> KR20120013462A </span> <span> . 2012-02-14</span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：念珠菌属南极来源的新型脂酶，具有增强的酶活性 </span> </p> </li> <li> <div> <b>2. </b><a class="enjiyixqcontent" href="/patent-detail/06130437711894.html">SYNTHESIS OF MEDIUM-CHAIN TRIGLYCERIDES BY GLYCEROLYSIS OF MEDIUM-CHAIN FATTY ACIDS USING AN IMMOBILIZED LIPASE FROM CANDIDA ANTARCTICA</a> <b>[P]</b> . <span> 外国专利： <!-- --> IN2006MU00311A </span> <span> . 2006-08-25</span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：南极假丝酵母的固定化脂酶通过甘油介导脂肪酸脂肪酸的合成来合成中链甘油三酸酯 </span> </p> </li> <li> <div> <b>3. </b><a class="enjiyixqcontent" href="/patent-detail/06130439899238.html">Enzymatic synthesis of citalopram derivative comprises diol treatment with acylating agent in presence of Candida antarctica lipase</a> <b>[P]</b> . <span> 外国专利： <!-- --> ES2228274A1 </span> <span> . 2005-04-01</span> </div> <p class="zwjiyix translation" style="max-width: initial;height: 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