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Analysis of force vector field during centrifugation for optimizing cell sheet adhesion

机译：用于优化细胞片粘附过程中的离心过程中力矢量场的分析

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Abstract >A three‐dimensional tissue was fabricated by layering cell sheets with centrifugation. In this system, an optimal centrifugal force promoted the adhesion between (a) a cell sheet and a culture dish, and (b) layered cell sheets, resulting in a significant decrease in the fabrication time of the tissue. However, negative effects like sliding/significant deformation of cell sheets were observed upon high rotational speed use. These negative effects inhibit the further shortening of the fabrication time. The sliding/deformation suggests that the centrifugal forces were applied on the cell sheets in unwanted directions. Studies on the force vector field applied to the object placed on the plate during centrifugation are not available, and thus, the reason for the occurrence of such negative effects is unclear. Here, we theoretically derived the spatial distribution of acceleration applied on a plate during centrifugation. Using this theory, we found that the negative effects were triggered by the centrifugal force in the direction parallel to the plate surface, which appeared due to an inclination of the plate surface against a horizontal plane. Therefore, by adding weights on the plate edge to maintain the plate surface in a horizontal position, we succeeded in eliminating the negative effects and in increasing the rotational speed, with the minimum risk of sliding/deformation of cell sheets. We succeeded in reducing the time to establish tight adhesion between a mouse myoblast sheet and a culture dish, and layered cell sheets by increasing the centrifugal force from 5 min to 1 min without significant cytotoxicity. </abstract> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> <div class="translation abstracttxt"> <span class="zhankaihshouqi fivelineshidden" id="abstract"> <span>机译：</span><Abstract Type =“Main”XML：Lang =“en”> <标题类型=“main”>抽象</ title> 通过分层细胞片具有离心的分层制造三维组织。在该系统中，最佳的离心力促进（a）蜂窝片和培养皿之间的粘合，和（b）层状细胞片，导致组织的制造时间的显着降低。然而，在高旋转速度使用时观察到像细胞片的滑动/显着变形的负面影响。这些负面影响抑制了制造时间的进一步缩短。滑动/变形表明离心力在不需要的方向上施加在细胞片上。在离心过程中，对施加到放置在板上的物体的力载体场的研究不可用，因此，发生这种负面影响的原因尚不清楚。在这里，我们理论上源自离心过程中施加在板上的加速度的空间分布。使用这种理论，我们发现，在平行于板表面的方向上由离心力触发负面影响，这由于板表面抵靠水平平面而出现。因此，通过在板边缘上添加重量以将板表面保持在水平位置，我们成功消除了负效应和增加旋转速度，具有蜂窝板的最小风险/变形的风险。我们通过在没有显着细胞毒性的情况下将离心力从5分钟增加到1分钟，减少了在小鼠肌细胞片和培养皿和培养皿之间建立紧密粘附的时间。</ p> </摘要> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> </div> <div class="record"> <h2 class="all_title" id="enpatent33" >著录项</h2> <ul> <li> <span class="lefttit">来源</span> <div style="width: 86%;vertical-align: text-top;display: inline-block;"> <a href='/journal-foreign-15005/'>《Biotechnology Progress》</a> <b style="margin: 0 2px;">|</b><span>2019年第5期</span><b style="margin: 0 2px;">|</b><span>共10页</span> </div> </li> <li> <div class="author"> <span class="lefttit">作者</span> <p id="fAuthorthree" class="threelineshidden zhankaihshouqi"> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Haraguchi Yuji&option=202" target="_blank" rel="nofollow">Haraguchi Yuji;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Kagawa Yuki&option=202" target="_blank" rel="nofollow">Kagawa Yuki;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Kubo Hirotsugu&option=202" target="_blank" rel="nofollow">Kubo Hirotsugu;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Shimizu Tatsuya&option=202" target="_blank" rel="nofollow">Shimizu Tatsuya;</a> </p> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zkzz" style="display: none;">展开▼</span> </div> </li> <li> <div style="display: flex;"> <span class="lefttit">作者单位</span> <div style="position: relative;margin-left: 3px;max-width: 639px;"> <div class="threelineshidden zhankaihshouqi" id="fOrgthree"> <p>Institute of Advanced Biomedical Engineering and ScienceTWIns Tokyo Women's Medical UniversityShinjuku‐ku Tokyo Japan;</p> <p>Ogino Memorial Laboratory Nihon Kohden CorporationTWInsShinjuku‐ku Tokyo Japan;</p> <p>Ogino Memorial Laboratory Nihon Kohden CorporationTWInsShinjuku‐ku Tokyo Japan;</p> <p>Institute of Advanced Biomedical Engineering and ScienceTWIns Tokyo Women's Medical UniversityShinjuku‐ku Tokyo Japan;</p> </div> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zhdw" style="display: none;">展开▼</span> </div> </div> </li> <li > <span class="lefttit">收录信息</span> <span style="width: 86%;vertical-align: text-top;display: inline-block;"></span> </li> <li> <span class="lefttit">原文格式</span> <span>PDF</span> </li> <li> <span class="lefttit">正文语种</span> <span>eng</span> </li> <li> <span class="lefttit">中图分类</span> <span><a href="https://www.zhangqiaokeyan.com/clc/15.html" title="生物科学">生物科学;</a></span> </li> <li class="antistop"> <span class="lefttit">关键词</span> <p style="width: 86%;vertical-align: text-top;"> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=3D tissue engineering&option=203" rel="nofollow">3D tissue engineering;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=acceleration vector&option=203" rel="nofollow">acceleration vector;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=attitude angle&option=203" rel="nofollow">attitude angle;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=deformation&option=203" rel="nofollow">deformation;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=rapid fabrication&option=203" rel="nofollow">rapid fabrication;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=sliding&option=203" rel="nofollow">sliding;</a> </p> <div class="translation"> 机译：3D组织工程;加速度向量;姿态角度;变形;快速制作;滑动; </div> </li> </ul> </div> </div> <div class="literature cardcommon"> <div class="similarity "> <h3 class="all_title" id="enpatent66">相似文献</h3> <div class="similaritytab clearfix"> <ul> <li class="active" >外文文献</li> <li >中文文献</li> <li >专利</li> </ul> </div> <div class="similarity_details"> <ul > <li> <div> <b>1. </b><a class="enjiyixqcontent" href="/academic-journal-cn_frontiers-structure-civil-engineering-english_thesis/0201291007696.html">Centrifuge model test and field measurement analysis for foundation pit with confined 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Chunlin DING</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Xiaohong MENG&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,Xiaohong MENG</a> <span> <a href="/journal-cn-9653/" target="_blank" rel="nofollow" class="tuijian_authcolor"> . 中国高等学校学术文摘·建筑与土木工程 </a> </span> <span> . 2009</span><span>,第003期</span> </span> </div> </li> <li> <div> <b>5. </b><a class="enjiyixqcontent" href="/academic-conference-cn_meeting-33983_thesis/020221003398.html">大方坯结晶器电磁搅拌力及磁场的有限元分析The FEM study of the stirring force andmagnetic fields Of EMS in</a> <b>[C]</b> <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor"> . </a> <span> <a href="/conference-cn-33983/" target="_blank" rel="nofollow" class="tuijian_authcolor"> . 第二届全国连铸电磁搅拌技术研讨会 </a> <span> <span> . 1998</span> </span> </div> </li> <li> <div> <b>6. </b><a class="enjiyixqcontent" href="/academic-degree-domestic_mphd_thesis/020312098526.html">Optimal Force Distribution and Robustness Analysis of Wheeled Mobile Robot based on Multy-objective Optimization</a> <b>[A] </b> <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Mahesh Kumar Isher&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor"> . Mahesh Kumar Isher</a> <span> . 2012</span> </span> </div> </li> </ul> <ul style="display: none;"> <li> <div> <b>1. </b><a class="enjiyixqcontent" href="/patent-detail/06130433677749.html">VECTOR HYSTERESIS MAGNETIC FIELD ANALYSIS METHOD, VECTOR HYSTERESIS MAGNETIC FIELD ANALYSIS PROGRAM, AND RECORDING MEDIUM</a> <b>[P]</b> . <span> 外国专利：  JP2008039709A </span> <span> . 2008-02-21</span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：矢量迟滞磁场分析方法，矢量迟滞磁场分析程序和记录介质 </span> </p> </li> <li> <div> <b>2. </b><a class="enjiyixqcontent" href="/patent-detail/06130434124964.html">Object e.g. heart, movement analysis implementing method for diagnosing heart disease, involves computing divergence value from vector field which is formed from displacement vectors for analysis of movement of object</a> <b>[P]</b> . <span> 外国专利：  DE102005061359A1 </span> <span> . 2007-07-05</span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：对象例如心脏，一种用于诊断心脏病的运动分析实现方法，涉及从由位移矢量形成的矢量场计算散度值，以分析物体的运动 </span> </p> </li> <li> <div> <b>3. </b><a class="enjiyixqcontent" href="/patent-detail/06130445875276.html">Correlation analysis method for image characteristics in corresponding real-time video images uses hybrid recursive technique for providing disparity vector field used as correlation vector field</a> <b>[P]</b> . <span> 外国专利：  DE10105423C1 </span> <span> . 2002-07-04</span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：对应的实时视频图像中图像特征的相关分析方法采用混合递归技术提供视差矢量场作为相关矢量场 </span> </p> </li> </ul> </div> </div> </div> <div class="theme cardcommon" style="overflow: auto;display:none"> <h3 class="all_title" id="enpatent55">相关主题</h3> <ul 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