Effects of cholinium‐based ionic liquids on Aspergillus niger lipase: Stabilizers or inhibitors

Nascimento Paloma A. M.; Picheli Flávio P.; Lopes André M.; Pereira Jorge F. B.; Santos‐Ebinuma Valéria C.

摘要

Abstract > Lipases are well‐known biocatalysts used in several industrial processes/applications. Thus, as with other enzymes, changes in their surrounding environment and/or their thermodynamic parameters can induce structural changes that can increase, decrease, or even inhibit their catalytic activity. The use of ionic compounds as solvents or additives is a common approach for adjusting reaction conditions and, consequently, for controlling the biocatalytic activity of enzymes. Herein, to elucidate the effects of ionic compounds on the structure of lipase, the stability and enzymatic activity of lipase from Aspergillus niger in aqueous solutions (at 0.05, 0.10, 0.50, and 1.00?M) of six cholinium‐based ionic liquids (cholinium chloride [Ch]Cl; cholinium acetate ([Ch][Ac]); cholinium propanoate ([Ch][Prop]); cholinium butanoate ([Ch][But]); cholinium pentanoate ([Ch][Pent]); and cholinium hexanoate ([Ch][Hex])) were evaluated over 24?hr. The enzymatic activity of lipase was maintained or enhanced in the lower concentrations of all the [Ch] + ‐ILs (below 0.1 M). [Ch][Ac] maintained the biocatalytic behavior of lipase, independent of the IL concentration and incubation time. However, above 0.1 M, [Ch][Pent] and [Ch][Hex] caused complete inhibition of the catalytic activity of the enzyme, demonstrating that the increase in the anionic alkyl chain length strongly affected the conformation of the lipase. The hydrophobicity and concentration of the [Ch] + ‐ILs play an important role in the enzyme activity, and these parameters can be controlled by adjusting the anionic alkyl chain length. The inhibitory effects of [Ch][Pent] and [Ch][Hex] may be of great interest to the pharmaceutical industry to induce pharmacological inhibition of gastric and pancreatic lipases. </abstract> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> <div class="translation abstracttxt"> <span class="zhankaihshouqi fivelineshidden" id="abstract"> <span>机译：</span><Abstract Type =“Main”XML：Lang =“en”> <标题类型=“main”>抽象</ title> > 脂肪酶是众所周知的生物催化剂，用于若干工业过程/应用。因此，与其他酶一样，其周围环境的变化和/或其热力学参数可以诱导可以增加，降低或甚至抑制它们催化活性的结构变化。作为溶剂或添加剂的使用离子化合物是调节反应条件的常见方法，并且因此用于控制酶的生物催化活性。在此，为了阐明离子化合物对脂肪酶结构，脂肪酶的稳定性和酶活性的影响曲霉尼日尔</ i> 在水溶液（0.05,0.10,0.50和1.00·m）的六种基于胆碱基的离子液体（氯化醋鎓氯化醋酸乙烯酯（乙酸缩水烯酮（[ch] [ac]）;丙啶丙烷（[ch] [prop ]）;丁醇丁酸胆氨酸（[CH] [但]）;戊烷戊烷（[CH] [PET]）;和己酸胆碱（[CH] [Hex]））以超过24μm。在所有[CH]的较低浓度下保持或增强脂肪酶的酶活性或增强 + </ sup> -ils（低于0.1米）。 [CH] [AC]保持脂肪酶的生物催化行为，与IL浓度和孵育时间无关。然而，高于0.1μm，[CH] [PET]和[六六角]引起酶催化活性的完全抑制，表明阴离子烷基链长度的增加强烈影响脂肪酶的构致酶。 [CH]的疏水性和浓度 + </ sup> - 在酶活性中起重要作用，可以通过调节阴离子烷基链长度来控制这些参数。 [CH] [PET]和[CH] [HEX]的抑制作用可能对制药行业感兴趣，以诱导胃和胰脂肪酶的药理学抑制。 </ p> </摘要> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> </div> <div class="record"> <h2 class="all_title" id="enpatent33" >著录项</h2> <ul> <li> <span class="lefttit">来源</span> <div style="width: 86%;vertical-align: text-top;display: inline-block;"> <a href='/journal-foreign-15005/'>《Biotechnology Progress》</a> <b style="margin: 0 2px;">|</b><span>2019年第5期</span><b style="margin: 0 2px;">|</b><span>共10页</span> </div> </li> <li> <div class="author"> <span class="lefttit">作者</span> <p id="fAuthorthree" class="threelineshidden zhankaihshouqi"> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Nascimento Paloma A. M.&option=202" target="_blank" rel="nofollow">Nascimento Paloma A. M.;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Picheli Flávio P.&option=202" target="_blank" rel="nofollow">Picheli Flávio P.;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Lopes André M.&option=202" target="_blank" rel="nofollow">Lopes André M.;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Pereira Jorge F. B.&option=202" target="_blank" rel="nofollow">Pereira Jorge F. B.;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Santos‐Ebinuma Valéria C.&option=202" target="_blank" rel="nofollow">Santos‐Ebinuma Valéria C.;</a> </p> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zkzz" style="display: none;">展开▼</span> </div> </li> <li> <div style="display: flex;"> <span class="lefttit">作者单位</span> <div style="position: relative;margin-left: 3px;max-width: 639px;"> <div class="threelineshidden zhankaihshouqi" id="fOrgthree"> <p>Department of Bioprocesses and Biotechnology School of Pharmaceutical SciencesS?o Paulo State University (UNESP)Araraquara S?o Paulo Brazil;</p> <p>Department of Bioprocesses and Biotechnology School of Pharmaceutical SciencesS?o Paulo State University (UNESP)Araraquara S?o Paulo Brazil;</p> <p>Department of Bioprocesses and Biotechnology School of Pharmaceutical SciencesS?o Paulo State University (UNESP)Araraquara S?o Paulo Brazil;</p> <p>Department of Bioprocesses and Biotechnology School of Pharmaceutical SciencesS?o Paulo State University (UNESP)Araraquara S?o Paulo Brazil;</p> <p>Department of Bioprocesses and Biotechnology School of Pharmaceutical SciencesS?o Paulo State University (UNESP)Araraquara S?o Paulo Brazil;</p> </div> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zhdw" style="display: none;">展开▼</span> </div> </div> </li> <li > <span class="lefttit">收录信息</span> <span style="width: 86%;vertical-align: text-top;display: inline-block;"></span> </li> <li> <span class="lefttit">原文格式</span> <span>PDF</span> </li> <li> <span class="lefttit">正文语种</span> <span>eng</span> </li> <li> <span class="lefttit">中图分类</span> <span><a href="https://www.zhangqiaokeyan.com/clc/15.html" title="生物科学">生物科学;</a></span> </li> <li class="antistop"> <span class="lefttit">关键词</span> <p style="width: 86%;vertical-align: text-top;"> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=choline&option=203" rel="nofollow">choline;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=enzymatic activity&option=203" rel="nofollow">enzymatic activity;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=inhibition&option=203" rel="nofollow">inhibition;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=ionic liquid&option=203" rel="nofollow">ionic liquid;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=lipase interaction&option=203" rel="nofollow">lipase interaction;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=stability&option=203" rel="nofollow">stability;</a> </p> <div class="translation"> 机译：胆碱;酶活性;抑制;离子液体;脂肪酶相互作用;稳定性; </div> </li> </ul> </div> </div> <div class="literature cardcommon"> <div class="similarity "> <h3 class="all_title" id="enpatent66">相似文献</h3> <div class="similaritytab clearfix"> <ul> <li class="active" >外文文献</li> <li 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Pedro&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Augusto Q. Pedro,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Patrícia Pereira&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Patrícia Pereira,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Maria J. Quental&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Maria J. Quental,</a> <span>-1</span> </span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：基于胆碱的Goods缓冲离子液体可作为重组小RNA的出色稳定剂和可回收的保存介质</span> </p> </li> <li> <div> <b>12. </b><a class="enjiyixqcontent" href="/open-access_resources_thesis/0100010715922.html ">Evaluation of temperature and moisture response surface on the lipase from pumpkin seeds fermentation using Aspergillus niger=Avaliação entre temperatura e umidade por superfície de resposta sobre lipase da fermentação de sementes de abóbora utilizando Aspergillus niger</a> <b>[O] </b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Luciana Cristina Lins de Aquino&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Luciana Cristina Lins de Aquino,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Cleide Mara Faria Soares&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Cleide Mara Faria Soares,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Kyzzes Barreto Araújo&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Kyzzes Barreto Araújo,</a> <span>2012</span> </span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：用黑曲霉评估南瓜种子发酵脂肪酶的温度和湿度响应表面=用黑曲霉评估南瓜种子发酵脂肪酶的每表面响应温度和湿度</span> </p> </li> <li> <div> <b>13. </b><a class="enjiyixqcontent" href="/ntis-science-report_de_thesis/020711396312.html">Radiation Effects Limits on Superconducting Magnets: Data Base for Copper Stabilizers</a> <b>[R] </b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Guinan, M. W.&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Guinan, M. W. </a> <span>1986</span> </span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：超导磁体的辐射效应限制：铜稳定器的数据库</span> </p> </li> </ul> <ul style="display: none;"> <li> <div> <b>1. </b><a class="enjiyixqcontent" href="/academic-degree-domestic_mphd_thesis/020315366201.html">Desulfurization of model oil by imidazolium based ionic liquid</a> <b>[A] </b> <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Nzinga Afonso Pedro&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor"> . Nzinga Afonso Pedro</a> <span> . 2019</span> </span> </div> </li> </ul> <ul style="display: none;"> <li> <div> <b>1. </b><a class="enjiyixqcontent" href="/patent-detail/06130412243804.html">Method for the production of lipase from Aspergillus niger using waste pulp processameto mangaba as substrate.</a> <b>[P]</b> . <span> 外国专利：  BR102014013453A2 </span> <span> . 2016-04-05</span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：以废纸浆加工阿曼陀巴为底物从黑曲霉生产脂肪酶的方法。 </span> </p> </li> <li> <div> <b>2. </b><a class="enjiyixqcontent" href="/patent-detail/06130425401181.html">NOVEL LIPASE FROM MUTATED STRAIN ASPERGILLUS NIGER AU15 FOR DETERGENT INDUSTRY</a> <b>[P]</b> . <span> 外国专利：  IN2010CH01105A </span> <span> . 2011-10-28</span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：突变菌株ASPERGILLUS NIGER AU15的新型脂酶，用于洗涤剂行业 </span> </p> </li> <li> <div> <b>3. </b><a class="enjiyixqcontent" href="/patent-detail/06130433455519.html">Stabilized composition and Stabilization Act of lipase from Aspergillus niger</a> <b>[P]</b> . <span> 外国专利：  JP4175696B2 </span> <span> . 2008-11-05</span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：黑曲霉脂肪酶的稳定组成和稳定作用 </span> </p> </li> </ul> </div> </div> </div> <div class="theme cardcommon" style="overflow: auto;display:none"> <h3 class="all_title" id="enpatent55">相关主题</h3> <ul id="subject"> </ul> </div> </div> </div> </div> <div class="right rightcon"> <div class="details_img cardcommon clearfix" style="margin-bottom: 10px;display:none;" > </div> </div> </div> <div id="thesis_get_original1" class="downloadBth" style="bottom: 19px;z-index: 999;" 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