A novel nicotinamide adenine dinucleotide control strategy for increasing the cell density of Haemophilus parasuis

Cheng Likun; Li Feng; Li Shuguang; Lin Chuwen; Fu Qiang; Yin Huanhuan; Tian Fengrong; Qu Guanggang; Wu Jiaqiang; Shen Zhiqiang

摘要

Abstract > Haemophilus parasuis is the causative agent of Gl?sser's disease and is a major source of economic losses in the swine industry each year. To enhance the production of an inactivated vaccine against H. parasuis , the availability of nicotinamide adenine dinucleotide (NAD) must be carefully controlled to ensure a sufficiently high cell density of H. parasuis . In the present study, the real‐time viable cell density of H. parasuis was calculated based on the capacitance of the culture. By assessing the relationship between capacitance and viable cell density/NAD concentration, the NAD supply rate could be adjusted in real time to maintain the NAD concentration at a set value based on the linear relationship between capacitance and NAD consumption. The linear relationship between cell density and addition of NAD indicated that 7.138?×?10 9 NAD molecules were required to satisfy per cell growth. Five types of NAD supply strategy were used to maintain different NAD concentration for H. parasuis cultivation, and the results revealed that the highest viable cell density (8.57, OD <sub>600</sub> ) and cell count (1.57?×?10 10 CFU/mL) were obtained with strategy III (NAD concentration maintained at 30?mg/L), which were 1.46‐ and 1.45‐ times more, respectively, than cultures with using NAD supply strategy I (NAD concentration maintained at 10 mg/L). An extremely high cell density of H. parasuis was achieved using this NAD supply strategy, and the results demonstrated a convenient and reliable method for determining the real‐time viable cell density relative to NAD concentration. Moreover, this method provides a theoretical foundation and an efficient approach for high cell density cultivation of other auxotroph bacteria. </abstract> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> <div class="translation abstracttxt"> <span class="zhankaihshouqi fivelineshidden" id="abstract"> <span>机译：</span><Abstract Type =“Main”XML：Lang =“en”> <标题类型=“main”>抽象</ title> > 嗜血杆菌parasuis </ i> 是GL的致病因子吗？SSER病的疾病，是每年猪行业经济损失的主要原因。增强灭活疫苗的生产 h。 Parasuis </ i> ，必须仔细控制烟酰胺腺嘌呤二核苷酸（NAD）的可用性，以确保足够高的细胞密度 h。 Parasuis </ i> 。在本研究中，实时活细胞密度 h。 Parasuis </ i> 基于培养物的电容计算。通过评估电容和活细胞密度/ NAD浓度之间的关系，可以实时调整NAD供应速率，以基于电容和NAD消耗之间的线性关系来维持在设定值下的NAD浓度。细胞密度与NAD的添加的线性关系表明7.138？×10 9 </ sup> 需要NAD分子以满足每个细胞生长。使用五种类型的NAD供应策略来维持不同的NAD浓度 h。 Parasuis </ i> 培养，结果显示，最高的活细胞密度（8.57，OD <sub> 600 </ sub> ）和细胞计数（1.57？×10 10 </ sup> 用策略III（保持在30μmg/ L）的策略III（NAD浓度）获得CFU / ml，分别比使用NAD供给策略I的培养物（NAD浓度为10 mg / L. ）。极高的细胞密度 h。 Parasuis </ i> 使用该NAD供应策略实现，结果表明了一种方便且可靠的方法，用于确定相对于NAD浓度的实时活细胞密度。此外，该方法提供了理论上的基础和一种高细胞密度培养的有效方法。 </ p> </摘要> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> </div> <div class="record"> <h2 class="all_title" id="enpatent33" >著录项</h2> <ul> <li> <span class="lefttit">来源</span> <div style="width: 86%;vertical-align: text-top;display: inline-block;"> <a href='/journal-foreign-15005/'>《Biotechnology Progress》</a> <b style="margin: 0 2px;">|</b><span>2019年第3期</span><b style="margin: 0 2px;">|</b><span>共9页</span> </div> </li> <li> <div class="author"> <span class="lefttit">作者</span> <p id="fAuthorthree" class="threelineshidden zhankaihshouqi"> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Cheng Likun&option=202" target="_blank" rel="nofollow">Cheng Likun;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Li Feng&option=202" target="_blank" rel="nofollow">Li Feng;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Li Shuguang&option=202" target="_blank" rel="nofollow">Li Shuguang;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Lin Chuwen&option=202" target="_blank" rel="nofollow">Lin Chuwen;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Fu Qiang&option=202" target="_blank" rel="nofollow">Fu Qiang;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Yin Huanhuan&option=202" target="_blank" rel="nofollow">Yin Huanhuan;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Tian Fengrong&option=202" target="_blank" rel="nofollow">Tian Fengrong;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Qu Guanggang&option=202" target="_blank" rel="nofollow">Qu Guanggang;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Wu Jiaqiang&option=202" target="_blank" rel="nofollow">Wu Jiaqiang;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Shen Zhiqiang&option=202" target="_blank" rel="nofollow">Shen Zhiqiang;</a> </p> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zkzz" style="display: none;">展开▼</span> </div> </li> <li> <div style="display: flex;"> <span class="lefttit">作者单位</span> <div style="position: relative;margin-left: 3px;max-width: 639px;"> <div class="threelineshidden zhankaihshouqi" id="fOrgthree"> <p>Post‐doctoral Scientific Research WorkstationShandong Binzhou Animal Science and Veterinary Medicine AcademyBinzhou China;</p> <p>Post‐doctoral Scientific Research WorkstationShandong Binzhou Animal Science and Veterinary Medicine AcademyBinzhou China;</p> <p>Post‐doctoral Scientific Research WorkstationShandong Binzhou Animal Science and Veterinary Medicine AcademyBinzhou China;</p> <p>Post‐doctoral Scientific Research WorkstationShandong Binzhou Animal Science and Veterinary Medicine AcademyBinzhou China;</p> <p>Post‐doctoral Scientific Research WorkstationShandong Binzhou Animal Science and Veterinary Medicine AcademyBinzhou China;</p> <p>Post‐doctoral Scientific Research WorkstationShandong Binzhou Animal Science and Veterinary Medicine AcademyBinzhou China;</p> <p>Post‐doctoral Scientific Research WorkstationShandong Binzhou Animal Science and Veterinary Medicine AcademyBinzhou China;</p> <p>Post‐doctoral Scientific Research WorkstationShandong Binzhou Animal Science and Veterinary Medicine AcademyBinzhou China;</p> <p>Institution of PoultryShandong Academy of Agricultural ScienceJinan China;</p> <p>Post‐doctoral Scientific Research WorkstationShandong Binzhou Animal Science and Veterinary Medicine AcademyBinzhou China;</p> </div> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zhdw" style="display: none;">展开▼</span> </div> </div> </li> <li > <span class="lefttit">收录信息</span> <span style="width: 86%;vertical-align: text-top;display: inline-block;"></span> </li> <li> <span class="lefttit">原文格式</span> <span>PDF</span> </li> <li> <span class="lefttit">正文语种</span> <span>eng</span> </li> <li> <span class="lefttit">中图分类</span> <span><a href="https://www.zhangqiaokeyan.com/clc/15.html" title="生物科学">生物科学;</a></span> </li> <li class="antistop"> <span class="lefttit">关键词</span> <p style="width: 86%;vertical-align: text-top;"> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=control strategy&option=203" rel="nofollow">control strategy;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Haemophilus parasuis&option=203" rel="nofollow">Haemophilus parasuis;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=linear relationship&option=203" rel="nofollow">linear relationship;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=nicotinamide adenine dinucleotide&option=203" rel="nofollow">nicotinamide adenine dinucleotide;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=real‐time monitoring&option=203" rel="nofollow">real‐time monitoring;</a> </p> <div class="translation"> 机译：控制策略;嗜血杆菌寄生;线性关系;烟酰胺腺嘌呤二核苷酸;实时监测; </div> </li> </ul> </div> </div> <div class="literature cardcommon"> <div class="similarity "> <h3 class="all_title" id="enpatent66">相似文献</h3> <div class="similaritytab clearfix"> <ul> <li class="active" >外文文献</li> <li >中文文献</li> <li >专利</li> </ul> </div> <div class="similarity_details"> <ul > <li> <div> <b>1. </b><a class="enjiyixqcontent" href="/academic-journal-cn_chinese-medical-journal_thesis/0201254471123.html">Pioglitazone inhibits the expression of nicotinamide adenine dinucleotide phosphate oxidase and p38 mitogen-activated protein kinase in rat mesangial cells</a> <b>[J]</b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=WANG Shan&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">WANG Shan,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=YE Shan-dong&option=202" 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Control of pentose phosphate-cycle activity by cellular requirement for reduced nicotinamide adenine dinucleotide phosphate</a> <b>[J]</b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=H. Kather&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">H. Kather,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=K. Brand&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">K. Brand,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=M. Rivera&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">M. Rivera </a> <a href="/journal-foreign-12650/" target="_blank" rel="nofollow" class="tuijian_authcolor">The biochemical journal .</a> <span>1972</span><span>,第5期</span> </span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：分离脂肪细胞中葡萄糖新陈代谢和脂肪生成的相互关系和控制。通过细胞要求对磷酸磷酸盐循环活性的控制减少的烟酰胺腺嘌呤二核苷酸磷酸酯的控制</span> </p> </li> <li> <div> <b>6. </b><a class="enjiyixqcontent" href="/academic-conference-foreign_meeting-209752_thesis/020514634327.html">Registration of damages of endothelial cell cultures by beta-nicotinamide adenine dinucleotide (NADH) fluorescence</a> <b>[C]</b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Werner Schramm&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Werner Schramm,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Forschungsinstitut fuer Molekulare Pharmakologie&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Forschungsinstitut fuer Molekulare Pharmakologie,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Berlin&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Berlin,</a> <a href="/conference-foreign-209752/" target="_blank" rel="nofollow" class="tuijian_authcolor">Optical and Imaging Techniques in Biomedicine .</a> <span>1995</span> </span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：用β-烟酰胺腺嘌呤二核苷酸（NADH）荧光记录内皮细胞培养物的损伤</span> </p> </li> <li> <div> <b>7. </b><a class="enjiyixqcontent" href="/academic-degree-foreign_mphd_thesis/02061909505.html">Neuroprotective Role of Nicotinamide Adenine Dinucleotide Precursor in Modulation of Mitochondrial Fragmentation and Brain Energy Metabolism</a> <b>[D] </b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Klimova, Nina.&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Klimova, Nina. </a> <span>2019</span> </span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：烟酰胺腺嘌呤二核苷酸前体在线粒体破碎和脑能代谢调节中的神经保护作用</span> </p> </li> <li> <div> <b>8. </b><a class="enjiyixqcontent" href="/academic-journal-foreign-pmc_canadian-journal-comparative-medicine_thesis/040002924370.html">Levels of nicotinamide adenine dinucleotide in extracellular body fluids of pigs may be growth-limiting for Actinobacillus pleuropneumoniae and Haemophilus parasuis</a> <b>[O] </b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Terence OReilly&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Terence OReilly,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Donald F. Niven&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Donald F. Niven </a> <span>2003</span> </span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：猪细胞外体液中烟酰胺腺嘌呤二核苷酸的水平可能限制胸膜肺炎放线杆菌和副猪嗜血杆菌的生长</span> </p> </li> <li> <div> <b>9. </b><a class="enjiyixqcontent" href="/open-access_resources_thesis/0100038026755.html ">Interrelationship and control of glucose metabolism and lipogenesis in isolated fat-cells. Control of pentose phosphate-cycle activity by cellular requirement for reduced nicotinamide adenine dinucleotide phosphate</a> <b>[O] </b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Kather, H.&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Kather, H.,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Rivera, M.&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Rivera, M.,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Brand, K.&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Brand, K. </a> <span>1972</span> </span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：分离的脂肪细胞中葡萄糖代谢和脂肪生成的相互关系和控制。通过减少还原烟酰胺腺嘌呤二核苷酸磷酸的细胞需求来控制磷酸戊糖循环活性</span> </p> </li> <li> <div> <b>10. </b><a class="enjiyixqcontent" href="/ntis-science-report_ad_thesis/020711160662.html">Hydrogen Peroxide And B-nicotinamide Adenine Dinucleotide Sensing AmperometricElectrodes Based on Electrical Connection of Horseradish Peroxidase Redox Centers To Electrodes Through a Three-dimensional Electron Relaying Polymer Network</a> <b>[R] </b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Vreeke, M.&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Vreeke, M.,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Maidan, R.&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Maidan, R.,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Heller, A.&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Heller, A. </a> <span>1992</span> </span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：过氧化氢和B-烟酰胺腺嘌呤二核苷酸检测安培电极基于辣根过氧化物酶氧化还原中心与电极的电连接通过三维电子中继聚合物网络</span> </p> </li> </ul> <ul style="display: none;"> <li> <div> <b>1. </b><a class="enjiyixqcontent" href="/academic-journal-cn_world-journal-gastroenterology-english_thesis/0201294146861.html">Induction of ischemic tolerance in rat liver via reduced nicotinamide adenine dinucleotide phosphate oxidase in Kupffer cells</a> <b>[J]</b> <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Kazuaki Tejima&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor"> . Kazuaki Tejima</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Masahiro Arai&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,Masahiro Arai</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Hitoshi Ikeda&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,Hitoshi Ikeda</a> <span> <a href="/journal-cn-52914/" target="_blank" rel="nofollow" class="tuijian_authcolor"> . 世界胃肠病学杂志：英文版 </a> </span> <span> . 2007</span><span>,第38期</span> </span> </div> </li> <li> <div> <b>2. </b><a class="enjiyixqcontent" href="/academic-journal-cn_infection-international-electronic-edition_thesis/0201265889633.html">Nicotinamide adenine dinucleotide phosphate promote the rapid growth of the tumor</a> <b>[J]</b> <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Sheng Hao&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor"> . Sheng Hao</a> <span> <a href="/journal-cn-38728/" target="_blank" rel="nofollow" class="tuijian_authcolor"> . 国际感染杂志（电子版） </a> </span> <span> . 2017</span><span>,第002期</span> </span> </div> </li> <li> <div> <b>3. </b><a class="enjiyixqcontent" href="/academic-journal-cn_infection-international-electronic-edition_thesis/0201265889622.html">Nicotinamide adenine dinucleotide phosphate promote the rapid growth of the tumor</a> <b>[J]</b> <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Sheng Hao1&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor"> . Sheng Hao1</a> <span> <a href="/journal-cn-38728/" target="_blank" rel="nofollow" class="tuijian_authcolor"> . 国际感染杂志（电子版） </a> </span> <span> . 2017</span><span>,第002期</span> </span> </div> </li> </ul> <ul style="display: none;"> <li> <div> <b>1. </b><a class="enjiyixqcontent" href="/patent-detail/06130412994206.html">Fuel cell, fuel cell production method, electronic device, nicotinamide adenine dinucleotide-immobilized electrode, nicotinamide adenine dinucleotide-immobilized carrier, enzyme reaction utilization apparatus, protein-immobilized electrode, and protein-immobilized carrier</a> <b>[P]</b> . <span> 外国专利：  JP5899885B2 </span> <span> . 2016-04-06</span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：燃料电池，燃料电池的制造方法，电子设备，烟酰胺腺嘌呤二核苷酸固定化电极，烟酰胺腺嘌呤二核苷酸固定化载体，酶反应利用装置，蛋白质固定电极和蛋白质固定化载体 </span> </p> </li> <li> <div> <b>2. </b><a class="enjiyixqcontent" href="/patent-detail/06130418001217.html">FUEL CELL, METHOD FOR PRODUCING FUEL CELL, ELECTRONIC APPARATUS, NICOTINAMIDE ADENINE DINUCLEOTIDE-IMMOBILIZED ELECTRODE, NICOTINAMIDE ADENINE DINUCLEOTIDE-IMMOBILIZED CARRIER, ENZYME REACTION UTILIZATION DEVICE, PROTEIN-IMMOBILIZED ELECTRODE AND PROTEIN-IMMOBILIZED CARRIER</a> <b>[P]</b> . <span> 外国专利：  US2014065494A1 </span> <span> . 2014-03-06</span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：燃料电池，燃料电池的制造方法，电子设备，烟酰胺腺嘌呤核糖体固定化的电极，烟酰胺腺嘌呤核糖体固定化的载体，酶反应利用电化装置，蛋白质和蛋白质固定化 </span> </p> </li> <li> <div> <b>3. </b><a class="enjiyixqcontent" href="/patent-detail/06130417102216.html">FUEL CELL, METHOD FOR MANUFACTURING FUEL CELL, ELECTRONIC APPARATUS, NICOTINAMIDE ADENINE DINUCLEOTIDE-IMMOBILIZED ELECTRODE, NICOTINAMIDE ADENINE DINUCLEOTIDE-IMMOBILIZED CARRIER, DEVICE USING ENZYME REACTION, PROTEIN-IMMOBILIZED ELECTRODE, AND PROTEIN-IMMOBILIZED CARRIER</a> <b>[P]</b> . <span> 外国专利：  EP2672559A1 </span> <span> . 2013-12-11</span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：燃料电池，制造燃料电池的方法，电子设备，烟酰胺腺嘌呤核糖体固定化的电极，烟酰胺腺嘌呤核糖体固定化的载体，使用酶反应的装置，蛋白质固定化和蛋白质固定化的装置 </span> </p> </li> </ul> </div> </div> </div> <div class="theme cardcommon" style="overflow: auto;display:none"> <h3 class="all_title" id="enpatent55">相关主题</h3> <ul id="subject"> </ul> </div> </div> </div> </div> <div class="right rightcon"> <div class="details_img cardcommon clearfix" style="margin-bottom: 10px;display:none;" > </div> </div> </div> <div id="thesis_get_original1" class="downloadBth" style="bottom: 19px;z-index: 999;" onclick="ywcd('0704028287809','4',7,2,1,'',this,24)" class="delivery" prompt="010401" title="通过人工服务将文献原文发送至邮箱" >获取原文</div> <div class="journalsub-pop-up" style="display: none"> <div class="journal-sub"> 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