Purification and biochemical characterization of an organic solvent‐tolerant and detergent‐stable lipase from Staphylococcus capitis

Rmili Fatma; Achouri Neila; Smichi Nabil; Krayem Najeh; Bayoudh Ahmed; Gargouri Youssef; Chamkha Mohamed; Fendri Ahmed

摘要

Abstract > A mesophilic bacterial culture, producing an extracellular alkaline lipase, was isolated from the gas‐washing wastewaters generated from the Sfax phosphate plant of the Tunisian Chemical Group and identified as Staphylococcus capitis strain. The lipase, named S. capitis lipase (SCL), has been purified to homogeneity from the culture medium. The purified enzyme molecular weight was around 45?kDa. Specific activities about 3,900 and 500?U/mg were measured using tributyrin and olive oil emulsion as substrates, respectively at 37°C and pH 8.5. Interestingly, the SCL maintained more than 60% of its initial activity over a wide pH values ranging from 5 to 11 with a high stability between pH 9 and 11 after 1 hr of incubation at room temperature. The lipase activity was enhanced in the presence of 2 mM of Mg 2+ , Ca 2+ , and K + . SCL showed significant stability in the presence of detergents and organic solvents. Altogether, these features make the SCL useful for industrial applications. Besides, SCL was compatible with commercially available detergents, and its incorporation increases lipid degradation performances making it a potential candidate in detergent formulation. </abstract> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> <div class="translation abstracttxt"> <span class="zhankaihshouqi fivelineshidden" id="abstract"> <span>机译：</span><Abstract Type =“Main”XML：Lang =“en”> <标题类型=“main”>抽象</ title> > 从突尼斯化学组的SFAX磷酸盐植物产生的气洗废水中分离出一种融化的细菌培养物，从突尼斯化学组的SFAX磷酸盐植物产生并被确定为葡萄球菌Capitis </ i> 拉紧。脂肪酶，命名 s。 Capitis </ i> 脂肪酶（SCL）已被纯化为来自培养基的均匀性。纯化的酶分子量约为45μlkda。在37℃和pH8.5分别使用Tricutyrin和橄榄油乳液作为基质测量约3,900和500的特定活动。有趣的是，SCL在室温下孵育1小时后，在5至11的宽pH值中保持超过5至11的初始活性的初始活性超过60％。脂肪酶活性在2mm的mg存在下提高 2 + </ sup> ，加利福尼亚州 2 + </ sup> 和K. + </ sup> 。 SCL在洗涤剂和有机溶剂存在下表现出显着的稳定性。完全，这些功能使SCL适用于工业应用。此外，SCL与市售的洗涤剂相容，其掺入增加了脂质降解性能，使其成为洗涤剂配方的潜在候选物。 </ p> </摘要> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> </div> <div class="record"> <h2 class="all_title" id="enpatent33" >著录项</h2> <ul> <li> <span class="lefttit">来源</span> <div style="width: 86%;vertical-align: text-top;display: inline-block;"> <a href='/journal-foreign-15005/'>《Biotechnology Progress》</a> <b style="margin: 0 2px;">|</b><span>2019年第4期</span><b style="margin: 0 2px;">|</b><span>共9页</span> </div> </li> <li> <div class="author"> <span class="lefttit">作者</span> <p id="fAuthorthree" class="threelineshidden zhankaihshouqi"> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Rmili Fatma&option=202" target="_blank" rel="nofollow">Rmili Fatma;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Achouri Neila&option=202" target="_blank" rel="nofollow">Achouri Neila;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Smichi Nabil&option=202" target="_blank" rel="nofollow">Smichi Nabil;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Krayem Najeh&option=202" target="_blank" rel="nofollow">Krayem Najeh;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Bayoudh Ahmed&option=202" target="_blank" rel="nofollow">Bayoudh Ahmed;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Gargouri Youssef&option=202" target="_blank" rel="nofollow">Gargouri Youssef;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Chamkha Mohamed&option=202" target="_blank" rel="nofollow">Chamkha Mohamed;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Fendri Ahmed&option=202" target="_blank" rel="nofollow">Fendri Ahmed;</a> </p> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zkzz" style="display: none;">展开▼</span> </div> </li> <li> <div style="display: flex;"> <span class="lefttit">作者单位</span> <div style="position: relative;margin-left: 3px;max-width: 639px;"> <div class="threelineshidden zhankaihshouqi" id="fOrgthree"> <p>Laboratoire de Biochimie et de Génie Enzymatique des LipasesENIS Route de SoukraSfax Tunisie;</p> <p>Laboratoire de Biochimie et de Génie Enzymatique des LipasesENIS Route de SoukraSfax Tunisie;</p> <p>Laboratoire de Biochimie et de Génie Enzymatique des LipasesENIS Route de SoukraSfax Tunisie;</p> <p>Laboratoire de Biochimie et de Génie Enzymatique des LipasesENIS Route de SoukraSfax Tunisie;</p> <p>Laboratoire de Génie Enzymatique et de MicrobiologieEcole Nationale d'Ingénieurs de SfaxSfax Tunisia;</p> <p>Laboratoire de Biochimie et de Génie Enzymatique des LipasesENIS Route de SoukraSfax Tunisie;</p> <p>Laboratoire des Bioprocédés Environnementaux P?le d'Excellence Régional AUF PER‐LBPECentre de Biotechnologie de SfaxSfax Tunisia;</p> <p>Laboratoire de Biochimie et de Génie Enzymatique des LipasesENIS Route de SoukraSfax Tunisie;</p> </div> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zhdw" style="display: none;">展开▼</span> </div> </div> </li> <li > <span class="lefttit">收录信息</span> <span style="width: 86%;vertical-align: text-top;display: inline-block;"></span> </li> <li> <span class="lefttit">原文格式</span> <span>PDF</span> </li> <li> <span class="lefttit">正文语种</span> <span>eng</span> </li> <li> <span class="lefttit">中图分类</span> <span><a href="https://www.zhangqiaokeyan.com/clc/15.html" title="生物科学">生物科学;</a></span> </li> <li class="antistop"> <span class="lefttit">关键词</span> <p style="width: 86%;vertical-align: text-top;"> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=alkaline lipase&option=203" rel="nofollow">alkaline lipase;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=detergent&option=203" rel="nofollow">detergent;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=isolation and characterization&option=203" rel="nofollow">isolation and characterization;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=organic solvent stability&option=203" rel="nofollow">organic solvent stability;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Staphylococcus capitis&option=203" rel="nofollow">Staphylococcus capitis;</a> </p> <div class="translation"> 机译：碱性脂肪酶;洗涤剂;分离和表征;有机溶剂稳定性;葡萄球菌Cacitis; </div> </li> </ul> </div> </div> <div class="literature cardcommon"> <div class="similarity "> <h3 class="all_title" id="enpatent66">相似文献</h3> <div class="similaritytab clearfix"> <ul> <li class="active" >外文文献</li> <li >中文文献</li> <li >专利</li> </ul> </div> <div class="similarity_details"> <ul > <li> <div> <b>1. </b><a class="enjiyixqcontent" href="/academic-journal-cn_journal-ocean-university-china_thesis/0201288753124.html">Identification and Characterization of a Novel Alkali-and High Temperature-Tolerant Lipase (Lip4346) from a Macroalgae-Associated Bacterial Strain</a> <b>[J]</b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=GU Xiaoqian&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">GU Xiaoqian,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=GUI Yuanyuan&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">GUI Yuanyuan,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=LI Jiang&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">LI Jiang,</a> <a href="/journal-foreign-4689/" target="_blank" rel="nofollow" class="tuijian_authcolor">中国海洋大学学报（英文版） .</a> <span>2021</span><span>,第001期</span> </span> </div> </li> <li> <div> <b>2. </b><a class="enjiyixqcontent" href="/journal-foreign-detail/0704019729577.html">Purification and characterization of an organic solvent-tolerant and detergent-stable lipase from Haloferax mediterranei CNCMM 50101</a> <b>[J]</b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Akmoussi-Toumi Siham&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Akmoussi-Toumi Siham,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Khemili-Talbi Souad&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Khemili-Talbi Souad,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Ferioune Imen&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Ferioune Imen,</a> <a href="/journal-foreign-20950/" target="_blank" rel="nofollow" class="tuijian_authcolor">International Journal of Biological Macromolecules: Structure, Function and Interactions .</a> <span>2018</span><span>,第期</span> </span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：Haloferax Mediterranei CNCMM 50101的有机溶剂耐受性和洗涤剂稳定脂肪酶的纯化和表征</span> </p> </li> <li> <div> <b>3. </b><a class="enjiyixqcontent" href="/journal-foreign-detail/0704013722126.html">Purification and Characterization of Organic Solvent-Stable Lipase from Organic Solvent-Tolerant Pseudomonas aeruginosa LST=03</a> <b>[J]</b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=OGINO HIROYASU&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">OGINO HIROYASU,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=NAKAGAWA SATOSHI&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">NAKAGAWA SATOSHI,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=SHINYA KAORI&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">SHINYA KAORI,</a> <a href="/journal-foreign-33725/" target="_blank" rel="nofollow" class="tuijian_authcolor">Journal of Bioscience and Bioengineering .</a> <span>2000</span><span>,第5期</span> </span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：耐有机溶剂性铜绿假单胞菌LST = 03的有机溶剂稳定脂肪酶的纯化和表征</span> </p> </li> <li> <div> <b>4. </b><a class="enjiyixqcontent" href="/academic-journal-foreign_detail_thesis/0204118618758.html">Purification and Characterization of Organic Solvent and Detergent Tolerant Lipase from Thermotolerant Bacillus sp. RN2</a> <b>[J]</b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Arda Pakpitcharoen&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Arda Pakpitcharoen,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Kenji Matsui&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Kenji Matsui,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Khajeenart Pothivejkul&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Khajeenart Pothivejkul,</a> <a href="/journal-foreign-21537/" target="_blank" rel="nofollow" class="tuijian_authcolor">International Journal of Molecular Sciences .</a> <span>2010</span><span>,第10期</span> </span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：耐热芽孢杆菌的有机溶剂和洗涤剂耐受脂肪酶的纯化与表征。 RN2</span> </p> </li> <li> <div> <b>5. </b><a class="enjiyixqcontent" href="/academic-conference-foreign_meeting-219989_thesis/020515188318.html">An Organic Solvent Tolerant Lipase from Serratia Marcescens ECU1010:Biochemical Characterization and Practical Application</a> <b>[C]</b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor"> </a> <a href="/conference-foreign-219989/" target="_blank" rel="nofollow" class="tuijian_authcolor">The 12th Asian Pacific Confederation of Chemical Engineering Congress(第十二届亚太化工联盟大会暨化工展览会)论文集 .</a> <span>2008</span> </span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：粘质沙雷氏菌ECU1010的耐有机溶剂脂肪酶：生化特性和实际应用</span> </p> </li> <li> <div> <b>6. </b><a class="enjiyixqcontent" href="/academic-degree-foreign_mphd_thesis/02061892614.html">Purification and Biophysical Characterization of Native Nicotinic Acetylcholine Receptor in Lipid-like Detergent Complexes</a> <b>[D] </b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=?Maldonado-Hernandez, Rafael&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">?Maldonado-Hernandez, Rafael </a> <span>2020</span> </span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：脂质样洗涤复合物中天然烟碱乙酰胆碱受体的纯化和生物物理表征</span> </p> </li> <li> <div> <b>7. </b><a class="enjiyixqcontent" href="/academic-journal-foreign-pmc_detail_thesis/04000965418.html">Purification and Characterization of Organic Solvent and Detergent Tolerant Lipase from Thermotolerant Bacillus sp. RN2</a> <b>[O] </b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Pornpimon Kanjanavas&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Pornpimon Kanjanavas,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Sintawee Khuchareontaworn&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Sintawee Khuchareontaworn,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Paisarn Khawsak&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Paisarn Khawsak,</a> <span>2010</span> </span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：耐热芽孢杆菌中有机溶剂和洗涤剂耐受脂肪酶的纯化与表征。 RN2</span> </p> </li> <li> <div> <b>8. </b><a class="enjiyixqcontent" href="/open-access_resources_thesis/01000104535390.html ">Purification and Characterization of Organic Solvent and Detergent Tolerant Lipase from Thermotolerant Bacillus sp. RN2</a> <b>[O] </b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Tadahiko Kajiwara&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Tadahiko Kajiwara,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Kosum Chansiri&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Kosum Chansiri,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Somchai Santiwatanakul&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Somchai Santiwatanakul,</a> <span>2010</span> </span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：耐热芽孢杆菌有机溶剂和洗涤剂耐受性脂肪酶的纯化和表征RN2</span> </p> </li> <li> <div> <b>9. </b><a class="enjiyixqcontent" href="/ntis-science-report_ad_thesis/020711772828.html">Purification and Characterization of Different Types of Exfoliative Toxin from 'Staphylococcus aureus'.</a> <b>[R] </b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Johnson, A. D.&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Johnson, A. D.,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext= Spero, L.&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor"> Spero, L.,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext= DeCicco, B. T.&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor"> DeCicco, B. T. </a> <span>1978</span> </span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：“金黄色葡萄球菌”不同类型脱落毒素的分离纯化与鉴定。</span> </p> </li> </ul> <ul style="display: none;"> <li> <div> <b>1. </b><a class="enjiyixqcontent" href="/academic-journal-cn_detail_thesis/0201295663726.html">Partial purification,immobilization and preliminary biochemical characterization of lipases from Rhizomucor pusillus</a> <b>[J]</b> <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Ana Lucia Ferrarezi&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor"> . Ana Lucia Ferrarezi</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Daniele H.Pivetta&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,Daniele H.Pivetta</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Gustavo Orlando Bonilla-Rodriguez&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,Gustavo Orlando Bonilla-Rodriguez</a> <span> <a href="/journal-cn-57737/" target="_blank" rel="nofollow" class="tuijian_authcolor"> . 酶研究进展（英文） </a> </span> <span> . 2013</span><span>,第4期</span> </span> </div> </li> <li> <div> <b>2. </b><a class="enjiyixqcontent" href="/academic-journal-cn_detail_thesis/0201297638162.html">A salt, detergent, and solvent tolerant protease from iBacillus/isp. B001: Low-cost, easy-purified, and enhanced production by raw material based culture strategy</a> <b>[J]</b> <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Jie Wu&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor"> . Jie Wu</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Aihua Deng&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,Aihua Deng</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Nana Shi&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,Nana Shi</a> <span> <a href="/journal-cn-56588/" target="_blank" rel="nofollow" class="tuijian_authcolor"> . 生命科学与技术进展（英文） </a> </span> <span> . 2013</span><span>,第12期</span> </span> </div> </li> <li> <div> <b>3. </b><a class="enjiyixqcontent" href="/academic-journal-cn_detail_thesis/0201295847913.html">iArthrobacter arilaitensis/iRe117 as a Source of Solvent-Stable Proteases: Production, Characteristics, Potential Application in the Deproteinization of Shrimp Wastes and Evaluation in Liquid Laundry Commercial Detergents</a> <b>[J]</b> <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Rayda Siala&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor"> . Rayda Siala</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Ines Hammemi&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,Ines Hammemi</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Sabrine Sellimi&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,Sabrine Sellimi</a> <span> <a href="/journal-cn-56588/" target="_blank" rel="nofollow" class="tuijian_authcolor"> . 生命科学与技术进展（英文） </a> </span> <span> . 2015</span><span>,第2期</span> </span> </div> </li> <li> <div> <b>4. </b><a class="enjiyixqcontent" href="/academic-journal-cn_detail_thesis/0201294713069.html">Purification and characterization of the kinetic parameters of cellulase produced from wheat straw by Trichoderma viride under SSF and its detergent compatibility</a> <b>[J]</b> <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Hafiz Muhammad Nasir Iqbal&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor"> . Hafiz Muhammad Nasir Iqbal</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Ishtiaq Ahmed&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,Ishtiaq Ahmed</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Muhammad Anjum Zia&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,Muhammad Anjum Zia</a> <span> <a href="/journal-cn-56588/" target="_blank" rel="nofollow" class="tuijian_authcolor"> . 生命科学与技术进展（英文） </a> </span> <span> . 2011</span><span>,第3期</span> </span> </div> </li> </ul> <ul style="display: none;"> <li> <div> <b>1. </b><a class="enjiyixqcontent" href="/patent-detail/06130403816592.html">In the presence of active and stable organic solvents in detergent, the preparation, purification and biochemical characteristics of a novel acid and heat-resistant SPPs protease serine produced by tolerant pleurisy strain ctm10057 were studied.</a> <b>[P]</b> . <span> 外国专利：  TN2017000473A1 </span> <span> . 2019-04-12</span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：在洗涤剂中存在活性和稳定的有机溶剂的情况下，研究了由耐受性胸膜炎菌株ctm10057产生的新型耐酸和耐热SPPs蛋白酶丝氨酸的制备，纯化和生化特性。 </span> </p> </li> <li> <div> <b>2. </b><a class="enjiyixqcontent" href="/patent-detail/06130415918654.html">Oxidant-containing wastewater treatment agent, oxidant-containing wastewater treatment method, oxidant-containing wastewater treatment device, organic solvent purification agent, organic solvent purification method, and organic solvent purification device</a> <b>[P]</b> . <span> 外国专利：  JP5629578B2 </span> <span> . 2014-11-19</span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：含氧化剂的废水处理剂，含氧化剂的废水处理方法，含氧化剂的废水处理装置，有机溶剂净化剂，有机溶剂净化方法以及有机溶剂净化装置 </span> </p> </li> <li> <div> <b>3. </b><a class="enjiyixqcontent" href="/patent-detail/06130423575614.html">Oxidant-containing wastewater treatment agent, oxidant-containing wastewater treatment method, oxidant-containing wastewater treatment device, organic solvent purification agent, organic solvent purification method, and organic solvent purification device</a> <b>[P]</b> . <span> 外国专利：  JPWO2010008072A1 </span> <span> . 2012-01-05</span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：含氧化剂的废水处理剂，含氧化剂的废水处理方法，含氧化剂的废水处理装置，有机溶剂净化剂，有机溶剂净化方法以及有机溶剂净化装置 </span> </p> </li> </ul> </div> </div> </div> <div class="theme cardcommon" style="overflow: auto;display:none"> <h3 class="all_title" id="enpatent55">相关主题</h3> <ul id="subject"> </ul> </div> </div> </div> </div> <div class="right rightcon"> <div class="details_img cardcommon clearfix" style="margin-bottom: 10px;display:none;" > </div> </div> </div> <div id="thesis_get_original1" class="downloadBth" style="bottom: 19px;z-index: 999;" onclick="ywcd('0704028287792','4',7,2,1,'',this,24)" class="delivery" prompt="010401" title="通过人工服务将文献原文发送至邮箱" >获取原文</div> <div class="journalsub-pop-up" style="display: none"> <div class="journal-sub"> <h2>期刊订阅</h2> <img src="https://cdn.zhangqiaokeyan.com/img/loginclose.png" alt="关闭" onclick="$('.journalsub-pop-up').hide()"> <p class="pardon">抱歉，该期刊暂不可订阅，敬请期待！</p> <p class="current">目前支持订阅全部北京大学中文核心（2020）期刊目录。</p> <div style="display: flex;margin-top: 69px;justify-content: space-between;"> <div class="no-sub" onclick="$('.journalsub-pop-up').hide()">暂不订阅</div> <div class="other-sub" onclick="continueSub('from=pc-detail')">继续订阅其他期刊</div> </div> </div> </div> <div class="right_btn"> <ul> <li class="gouwuche">  </li> <li class="yijian"> <a href="javascript:void(0);" onclick="link_analysis('/mycenter/auth/complaint.html',this)" title="意见反馈">意见反馈</a> </li> <li class="top"> <a href="javascript:scrollTo(0,0);" title="回到顶部">回到顶部</a> </li> <li class="shouye"> <a href="/" title="首页">回到首页</a> </li> </ul> </div> <div class="xllindexfooter"> <div class="xllindexfootercenter"> <div class="xllindexfooterleft left" > <div class="xllindexfooterleftli"> <ul> <li><a href="/about.html" title="关于掌桥">关于掌桥</a></li> <li><a href="/help/helpmap.html" title="资源导航">资源导航</a></li> <li><a href="/help/helpguide.html" title="新手指南">新手指南</a></li> <li><a href="/help/helpcenter.html" title="常见问题">常见问题</a></li> <li><a href="/sitemap.html" title="网站地图">网站地图</a></li> <li><a href="/help/helpcenter.html?type=9" title="版权声明">版权声明</a></li> </ul> </div> <div class="xllindexfooterleft"> <p class="xllindexfooterlefteamil">客服邮箱：kefu@zhangqiaokeyan.com</p> <div class="xllindexfooterlefttcp"> <div class="xllindexfooterpoliceiimg"></div> <div class="xllindexfooterpoliceispan"> <span>京公网安备：11010802029741号 </span> <span>ICP备案号：<a href="https://beian.miit.gov.cn" rel="nofollow" target="_blank" title="ICP备案号">京ICP备15016152号-6</a></span> <span>六维联合信息科技 (北京) 有限公司©版权所有</span> </div> </div> </div> </div> <div class="xllindexfooterright left"> <ul> <li> <p style="font-weight: bold;">客服微信</p> <div></div> </li> <li> <p style="font-weight: bold;">服务号</p> <div></div> </li> </ul> </div> </div> </div> <span id="0704028287792down" data-source="7," data-out-id="FdzMZP/VzQDSHsYq2SpSCEH/kJmzOAXUruRnXFdhvXUjA+wbjAOMF3gCTuuuxYjW," data-f-source-id="7" data-title="Purification and biochemical characterization of an organic solvent‐tolerant and detergent‐stable lipase from Staphylococcus capitis" data-price="20" data-site-name="" data-transnum="24" style="display:none;"></span> <input type="hidden" value="4" id="sourcetype"> <input type="hidden" value="15005" id="journalid"> <input type="hidden" value="1" id="inyn_provide_service_level"> <input type="hidden" value="https://cdn.zhangqiaokeyan.com" id="imgcdn"> <input type="hidden" value="1" id="isdeatail"> <input type="hidden" value="" id="syyn_indexed_database"> <input type="hidden" value="" id="servicetype"> <input type="hidden" id="pagename" value="Purification and biochemical characterization of an organic solvent‐tolerant and detergent‐stable lipase from Staphylococcus capitis"/> <input type="hidden" value="thesis_get_original" id="pageIdentification"> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/jquery-1.12.4.js"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/common/lwlh_ajax.js"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/zq.js?v=5.7.8"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/common/common.js?v=5.7.8"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/jquery.cookie.js"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/top.js?v=5.7.8"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/common/tip.js?v=5.7.8"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/common/login.js?v=5.7.8"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/common/down.js?v=5.7.8"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/common/search.js?v=5.7.8"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/common/newmail.js?v=5.7.8"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/searchtype.js?v=5.7.8"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/user/regist.js?v=5.7.8"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/zxs_solor/detail.js?v=5.7.8"></script> <script type="text/javascript" src="https://www.zhangqiaokeyan.com/statistics/static/pagecollection.js"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/tj.js"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/common/sse.js?v=5.7.8"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/pushbaidu.js"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/common/history.js"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/util/cookie.js?v=5.7.8"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/weipu/weipu.js?v=5.7.8"></script> </body> <script> $(function(){ var weiPuStatus = getCookie('WeiPuStatus'); if(weiPuStatus){ tipWeiPuStatus(weiPuStatus); delCookie('WeiPuStatus'); } getFacetKeywordVoInId(); var sourcetype = $("#sourcetype").val(); var inyn_provide_service_level = $("#inyn_provide_service_level").val(); if((sourcetype ==1||sourcetype==4)&&inyn_provide_service_level!=4){ getJournal(); } }) </script> </html>