A zeolite as a tool for successful refolding of PEGylated proteins and their reassembly with tertiary structures

Sonotaki Seiichi; Noguchi Keiichi; Yohda Masafumi; Murakami Yoshihiko

摘要

Abstract >In the present study, we demonstrated zeolites' potential contribution to establish a method for preparing successfully refolded and reassembled PEGylated protein nanoparticles without the use of protein denaturants through the proteins' reassembly process. At first, the PEGylated nanoparticles are disassembled into identical PEGylated protein subunits by means of protein denaturants, and then the denatured subunits are adsorbed to zeolites. After the complete removal of denaturants, high‐molecular‐weight poly(ethylene glycol) (PEG) molecules are added to a solution where the zeolites suspend. Consequently, the PEGylated proteins are gradually reassembled into nanoparticles because the subunits are desorbed from the zeolites by the steric hindrance of the added PEG molecules. The present study reveals that PEGylated encapsulin was reassembled and hollow encapsulin nanoparticles were obtained. The results clearly demonstrate the usefulness of zeolites as a tool for the successful refolding of PEGylated proteins and their reassembly with tertiary structures. </abstract> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> <div class="translation abstracttxt"> <span class="zhankaihshouqi fivelineshidden" id="abstract"> <span>机译：</span><Abstract Type =“Main”XML：Lang =“en”> <标题类型=“main”>抽象</ title> 在本研究中，我们证明了沸石的潜在贡献，以建立制备成功重折叠和重新组装的聚乙二醇化蛋白纳米颗粒的方法，而不通过蛋白质的重新组装方法使用蛋白质变性剂。首先，将聚乙烯化纳米颗粒通过蛋白质变性剂分解成相同的聚乙二醇化蛋白质亚基，然后将变性亚基吸附到沸石中。在完全去除变性剂后，将高分子量聚（乙二醇）（PEG）分子加入到沸石悬浮的溶液中。因此，将聚乙二醇化的蛋白质逐渐将其重新组装成纳米颗粒，因为亚基通过添加的PEG分子的空间障碍从沸石中解吸。本研究表明，重新组装了聚乙二醇化的包封蛋白，并获得中空的包封蛋白纳米颗粒。结果清楚地证明了沸石作为成功重新折叠聚乙二醇化蛋白的工具的工具及其与三级结构的重新组装。</ p> </摘要> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> </div> <div class="record"> <h2 class="all_title" id="enpatent33" >著录项</h2> <ul> <li> <span class="lefttit">来源</span> <div style="width: 86%;vertical-align: text-top;display: inline-block;"> <a href='/journal-foreign-15005/'>《Biotechnology Progress》</a> <b style="margin: 0 2px;">|</b><span>2019年第5期</span><b style="margin: 0 2px;">|</b><span>共11页</span> </div> </li> <li> <div class="author"> <span class="lefttit">作者</span> <p id="fAuthorthree" class="threelineshidden zhankaihshouqi"> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Sonotaki Seiichi&option=202" target="_blank" rel="nofollow">Sonotaki Seiichi;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Noguchi Keiichi&option=202" target="_blank" rel="nofollow">Noguchi Keiichi;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Yohda Masafumi&option=202" target="_blank" rel="nofollow">Yohda Masafumi;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Murakami Yoshihiko&option=202" target="_blank" rel="nofollow">Murakami Yoshihiko;</a> </p> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zkzz" style="display: none;">展开▼</span> </div> </li> <li> <div style="display: flex;"> <span class="lefttit">作者单位</span> <div style="position: relative;margin-left: 3px;max-width: 639px;"> <div class="threelineshidden zhankaihshouqi" id="fOrgthree"> <p>Department of Organic and Polymer Materials ChemistryTokyo University of Agriculture and TechnologyTokyo Japan;</p> <p>Instrumentation Analysis CenterTokyo University of Agriculture and TechnologyTokyo Japan;</p> <p>Department of Biotechnology and Life ScienceTokyo University of Agriculture and TechnologyTokyo Japan;</p> <p>Department of Organic and Polymer Materials ChemistryTokyo University of Agriculture and TechnologyTokyo Japan;</p> </div> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zhdw" style="display: none;">展开▼</span> </div> </div> </li> <li > <span class="lefttit">收录信息</span> <span style="width: 86%;vertical-align: text-top;display: inline-block;"></span> </li> <li> <span class="lefttit">原文格式</span> <span>PDF</span> </li> <li> <span class="lefttit">正文语种</span> <span>eng</span> </li> <li> <span class="lefttit">中图分类</span> <span><a href="https://www.zhangqiaokeyan.com/clc/15.html" title="生物科学">生物科学;</a></span> </li> <li class="antistop"> <span class="lefttit">关键词</span> <p style="width: 86%;vertical-align: text-top;"> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=drug carrier&option=203" rel="nofollow">drug carrier;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=encapsulin&option=203" rel="nofollow">encapsulin;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=hollow nanoparticle&option=203" rel="nofollow">hollow nanoparticle;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=PEGylation&option=203" rel="nofollow">PEGylation;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=zeolite&option=203" rel="nofollow">zeolite;</a> </p> <div class="translation"> 机译：药物载体;包封剂;中空纳米粒子;聚乙二醇化;沸石; </div> </li> </ul> </div> </div> <div class="literature cardcommon"> <div class="similarity "> <h3 class="all_title" id="enpatent66">相似文献</h3> <div class="similaritytab clearfix"> <ul> <li class="active" >外文文献</li> <li >中文文献</li> <li >专利</li> </ul> </div> <div class="similarity_details"> <ul > <li> <div> <b>1. </b><a class="enjiyixqcontent" href="/academic-journal-cn_journal-central-south-university_thesis/020128320937.html">Tertiary structure-based protein classification by virtual-bond-angles series</a> <b>[J]</b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=LI Bin&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">LI Bin,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=HE Hong-bo&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">HE Hong-bo,</a> <a 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