Highly Stable Pyrimidine-Motif Triplex Formation at Physiological pH Values by a Bridged Nucleic Acid Analogue

摘要

Formation of a stable triplex DNA molecule at physiological pH values is a highly desirable phenomenon in molecular biology and medicinal chemistry because of its great importance in regulation of gene expression,site-specific cleavage of DNA,gene mapping and isolation,maintenance of folded chromosome conformations,and gene-targeted mutagenesis.In a pyrimidine-motif triplex DNA,the (homopyrimi-dine) triplex-forming oligonucleotide (TFO) binds with the homopurine tract of the target duplex DNA in a sequence-specific manner through Hoogsteen hydrogen bonds to form T centre dot A:T and C+ centre dot G:C triads.