Disaccharides as Sialic Acid Aldolase Substrates: Synthesis of Disaccharides Containing a Sialic Acid at the Reducing End

摘要

Sialic acid aldolases (EC 4.1.3.3), or N-acetylneuraminate lyases (Neu5Ac lyases or NALs), catalyze the reversible aldol cleavage of N-acetylneuraminic acid (Neu5Ac) to form pyruvate and N-acetyl-D-mannosamine (ManNAc) (Scheme 1). They have been extensively studied and the reverse reaction has been widely applied in the enzymatic synthesis of sialic acids and their derivatives.