Detection of Genetic Variation in the Wild Populations of Indian Major Carps Using Random Amplified Polymorphic DNA Fingerprinting

摘要

Genetic variations in wild populations of Indian Major Carps (IMCs) from six geographically isolated locations of Central India Rivers were examined using Randomly Amplified Polymorphic DNA (RAPD). Thirty six specimens of each species from each location were collected. Genomic DNA was isolated from the liver tissues. Out of twenty RAPD primers used, thirteen were found to be scorable on agarose gel, of which 83.33% were polymorphic for Laheo rohita, 80.0% for Cirrhinus mrigala and 75.0% for Catla catla. A total of 576 RAPD bands were amplified. Dendrogram generated for species-specific genetic evaluation revealed that C. catla was genetically dissimilar from L. rohita and C. mrigala. The present investigation is the first report on wild varieties of IMCs while the available data on farmed varieties suggest that C catla and C mrigala are more genetically similar to each other than to L. rohita. This study also revealed high intra-specific genetic variation in the wild populations of IMCs. To conclude, the present study suggest high levels of genetic variation and population differentiation required for dynamic evolution and RAPD assay therefore may have potential use for establishing genetic relationship, genome specificity and phylogeny among wild species of IMCs.