A Tailor-Made Chimeric Thiamine Diphosphate Dependent Enzyme for the Direct Asymmetric Synthesis of (S)-Benzoins

摘要

Thiamine diphosphate dependent enzymes are well known for catalyzing the asymmetric synthesis of chiral a-hvdroxy ketones from simple prochiral substrates. The steric and chemical properties of the enzyme active site define the nroduct spectrum. Enzymes catalyzing the carboligation of aromatic aldehydes to (S)-benzoins have not so far been identified-We were able to close this gap by constructing a chimeric enzyme, which catalyzes the synthesis of various (S)-benzoins with excellent enantiomeric excess (>99 %) and very good conversion.