Bound Cations Significantly Stabilize the Structure of Multiprotein Complexes in the Gas Phase

摘要

Mass spectrometry (MS) has revealed the composition, stoichiometry, connectivity, and dynamics of many multi-protein complexes that remain challenging for other structural biology methods. More recently, ion mobility (IM), a gas-phase separation technique that resolves protein ions according to their size and charge, coupled with MS (IM-MS) has been used to generate three-dimensional structure information from these samples.