Far-Field Nanoscopy with Reversible Chemical Reactions

摘要

Fluorescence microscopy is a powerful standard tool used to study structures and dynamics in living cells. Its routine application in cell biology has been boosted by the development of genetically encoded fluorescent tags that can be fused to the gene of the target protein and expressed in living cells.'1' However, optical diffraction limits the resolution of far-field microscopy to roughly 200 nm in the lateral plane and around 500 nm along the axis of observation which is 1-2 orders of magnitude larger than many of the macromolecular complexes and clusters of interest.