Solid-State NMR Spectroscopy Reveals that E. coli Inclusion Bodies of HET-s(218-289) are Amyloids

摘要

Heterologous protein expression in E. coli has taken on tremendous importance in many fields of biology since it allows the preparation of sufficient amounts of sample for physicochemical investigations.Often, however, expressed proteins are deposited as insoluble aggregates, the so-called inclusion bodies (IBs).The formation of IBs-dense intracellular deposits of amorphous appearance that can reach 1 μm~3 in volume-is often regarded as an obstacle that prevents the isolation of native heterologous proteins from E. coli cells.