Mass-production of Fungivorous Nematode, Aphelenchus avenae BASTIAN 1865, on Industrial Vegetable/Animal Wastes

摘要

The fungivorous nematode, Aphelenchus avenae, can be mass-produced by feeding Rhlzoctonia solani growing on solid substrates composed of various industrial vegetable wastes in different combinations and ratios. Supplementation of broiler process slurry waste as one of the ingredient with vegetable wastes greatly improved nematode multiplication. The addition of 30% (w/w dry weight) used green tea leaves significantly increased A. avenae propagation by maintaining favourable pH. The highest multiplication of A. avenae 113.8 x 10~4 was obtained 20 days after inoculation with the initial 200 nematodes on the 20 g substrates consisted of 35% broiler process slurry waste, 35% distilled spirit strained lees, and 30% used green tea leaves moistened with 60% water. From the same initial population but 30 days after inoculation, the second highest multiplication was 92.7 x 10~4 on the substrate consisted of 35% distilled spirit strained lees, 35% orange juice strained lees, and 30% used green tea leaves. A.avenae was also mass-cultured on sponge substrates permeated with PDA solution as nutrient source for the fungus R. solani. The highest multiplication (109 x 10~4) was recorded on 20 g substrates (sponge 1 g, nutrient solution 16 ml) permeated by potatodextrose agar at 25°C on 30 days after inoculation with 200 nematodes.