Inhibitory effect of high NH_4~+-N concentration on anaerobic biotreatment of fresh leachate from a municipal solid waste incineration plant

摘要

Fresh leachate from municipal solid waste (MSW) incineration plants generally contains extremely high NH_4~+-N concentration which could inhibit the bioactivity of microorganisms. The inhibitory effect of high NH_4~+-N concentration on anaerobic biotreatment of fresh leachate from a MSW incineration plant in China has been investigated in this study. The inhibition processes was studied by both static tests and a laboratory-scale expanded granular sludge bed (EGSB) reactor. The specific methanogenic activity (SMA) of the microorganisms in anaerobic granular sludge was inhibited with the NH_4~+-N concentration increasing to 1000 mg/L in static tests. As well the chemical oxygen demand (COD) removal efficiency and the methane yield decreased in the EGSB reactor, while the volatile fatty acids (VFAs) accumulated and extracellular polymeric substances (EPS) of the anaerobic granular sludge increased with NH_4~+-N concentration rising to 1000 mg/L, without any rebounding during 30 days of operation. Decreasing NHJ-N concentration to 500 mg/L in influent, the COD removal efficiency recovered to about 85% after 26 days. 1000 mg/L of NH_4~+-N in leachate was suggested to be the inhibition threshold in EGSB reactor. High-throughput sequencing results showed little changes in microbial communities of the sludge for a high NH_4~+-N concentration, indicating that the survival of most microorganisms was not affected under such a condition. It inhibited the bioactivity of the microorganisms, resulting in decrease of the COD removal efficiency.