DNA LIBRARY QUANTIFICATION KIT

摘要

The new ddPCR Library Quantification Kit for IlluminaTruSeq sample preparation kits enables QX100 Droplet Digital PCR system users to precisely and directly measure amplifiable library concentrations for next generation sequencing. The TruSeq sample preparation method is the technology behind Illumina's MiSeq and HiSeq next generation sequencing platforms. Using the ddPCR Library Quantification Kit to quantify TruSeq DNA libraries maximizes the number of useable reads, enables consistent loading, and optimizes the utilization of every sequencing run. The resulting data provides additional measures of library quality not provided by other methods, including the percentage of nonamplifiable species such as adapter dimers as well as the size range of library inserts.