Localization of Sry gene on Y chromosome of Muntjac munticus vaginalis

摘要

The chromosomes 1, Y_1, Y_2 of Muntiac munticus vaginalis were isolated by flurescence activated chromosome sorting and amplified by degenerate oligonucleotide primed-polymerase chain reaction (DOP-PCR). A primer pair within human Sry HMG box was designed and the Sry gene of the male M. m vaginalis was amplified. The product was cloned and sequenced. The result proved that Sry is located on chromosome Y_2, which is the sex-determin- ing chromosome in the male M. m vaginalis. primer pair