Characterization of the separate kinase domain of chicken liver 6-phosphofructo-2-Kinase/fructose-2, 6-bisphosphatase

摘要

The bifunctional enzyme 6-phosphofructo-2-kinase/fructose-2, 6,-bisphosphatase consists of two dis- tinct domains which catalyze the synthesis and hydrolysis of fructose-2, 6-bisphosphate, respectively. In this work the properties of the separate 6-phosphofructo-2-kinase domain were investigated. Purification of the expressed separate do- main or isolation of this domain from purified glutathione S-transferase (GST) fusion protein with thrombin cleavage led to the loss of its kinase activity.