Genetic mapping of telomere-associated sequences in soybean (Glycine max)

摘要

Two telomere-associated sequences (TAS), named STAS8 and STAS10, were cloned from soybean genomic DNA using polymerase chain reaction (PCR) amlification. Southern analysis showed that they were sequeences with moderate copy number in soybean genome. Sequence analysis demonstrated that STAS10 had tandemly arrayed con- sensus sequences of TTTAGGG and TTAGGG. The mapping of these two TAS was performed with a population of F8 re- combinant inbred line using restriction fragment length polymorphisms (RFLP).