Effects of Vitamins A and E on TiO_2 Nanoparticles-lnduced Spermatogenesis Defects in Male Wistar Rats

摘要

This report was aimed to study the validity of vitamins A and E on the spermatogenesis disorders caused by titanium dioxide nanoparticles (TiO2 NPs). Thirty-six male Wistar rats were randomly divided into six groups. Two groups as controls received bi-distilled water and olive oil for three weeks, respectively. The third group treated with 300 mg/kg TiO2 NPs for two weeks. The other groups received vitamin E (100 IU/kg), vitamin A (100 IU/kg), and the combination of these vitamins for 3 weeks, respectively. At the end of the study, sperm parameters, sperm chromatin integrity, and testicular histopathology changes were assessed. Sperm count, motility, and viability significantly decreased in TiO2 NPs-intoxicated rats (p 0.05). Sperm chromatin staining also showed that spermatozoa with the impaired chromatin were upper in the nanoparticle receiving group matched to the controls (p 0.05). Histopathological lesions like inflammation were found in the group treated by TiO2 NPs compared to the controls (p 0.05). Accordingly, vitamins E and A could significantly prevent these changes (p 0.05). The combination of the two vitamins showed a better performance than the separate administration of each one. The results of this study illustrated that vitamins E and A have a potent protective effect against testicular toxicity.