Some properties of C-phycocyanin from a native strain Anabaena affinis isolated from Kovada Lake, Isparta, Turkey

摘要

Some properties of C-Phycocyanin (C-PC) from a native strain Anabaena affinis isolated from Kovada Lake, Isparta, Turkey were discussed in the present. Purification process was resulted in analytical grade product with 6.93-fold increase in phycocyanin purity. By using UV-VIS and fluorescence spectrophotometry, the highest absorbance and fluorescence were determined as 620 and 646 nm, respectively. SDS-PAGE analysis revealed the presence of two major bands with a molecular mass of 17.1 and 18.6 kDa corresponding to the a and 13 subunits and as confirmed by MALDI-TOF mass spectrometric analysis. Molecular mass of pure C-PC was found as 156.2 and 198 kDa by HPLC gel filtration. Aggregation states of C-PC were estimated as hexameric-(alpha beta)(6) and trimeric forms-(alpha beta)(3) along with two binding polypeptides of 27 kDa. High antioxidant activity was determined at 480 mu M TEAC/mg phycocyanin while no antimicrobial activity was detected.