Long noncoding RNA CASC15 is upregulated in glioma and facilitates cell proliferation and metastasis via targeting miR-130b-3p

摘要

OBJECTIVE: Recent studies have discovered a class of long non-coding RNAs (lncRNAs) which are dysregulated in various tumors and linked to carcinogenesis. This paper aimed to uncover the molecular functions of lncRNA CASC15 in glioma tumorigenesis. PATIENTS AND METHODS: Real Time-quantitative Polymerase Chain Reaction (RT-qPCR) was performed to detect CASC15 expression in 50 glioma samples and 4 glioma cells. Besides, proliferation assay, transwell assay and wound healing assay were performed to explore the role of CASC15 in glioma progression in vitro. Furthermore, the interaction between CASC15 and miR-130b-3p in glioma was studied by performing the Dual-Luciferase reporter assay. In addition, tumor formation and metastasis assay were performed in vivo. RESULTS: CASC15 expression was remarkably upregulated in glioma samples compared with that in adjacent samples. Cell proliferation, invasion and migration in glioma were inhibited via knockdown of CASC15 in vitro. Moreover, miR-130b-3p was upregulated via knockdown of CASC15 in vitro. Besides, miR-130b-3p was a direct target of CASC15 in glioma. Tumor formation and metastasis were inhibited after CASC15 was knockdown in vivo. CONCLUSIONS: These results suggest that CASC15 could repress metastasis and proliferation of glioma by sponging miR-130b-3p in vitro and in vivo, which may offer a new therapeutic intervention for glioma patients.