Rapid Filter-Based Detection and Culture of Burkholderia pseudomallei from Small Volumes of Urine

摘要

Melioidosis is an infectious disease with a high case fatality rate in humans that is caused by the Gram-negative, soil-dwelling bacterium Burkholderia pseudomallei (1–5). Common to Southeast Asia and northern Australia, melioidosis is recognized as endemic in 48 equatorial-zone nations (6, 7). A 2015 study suggests that melioidosis cases are underreported in regions of endemicity and estimates that 165,000 cases occur annually, leading to 89,000 deaths (6). As a result of global travel, melioidosis can also be diagnosed in regions where it is not endemic. A recent review by Michael Dan indicates that 72 melioidosis cases in travelers returning from regions of endemicity were reported in the literature through July 2015 (8).