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首页> 外文期刊>Journal of Clinical Microbiology >Real-Time PCR Assay for Identification of Blastomyces dermatitidis in Culture and in Tissue
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Real-Time PCR Assay for Identification of Blastomyces dermatitidis in Culture and in Tissue

机译:实时PCR检测鉴定培养物中和组织中的皮肤芽孢杆菌

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The TaqMan real-time PCR assay was developed from the Blastomyces dermatitidis BAD1 gene promoter. The assay identified all haplotypes of B. dermatitidis and five of six positive paraffin-embedded tissues. The assay sensitivity threshold was 1 pg genomic DNA of the mold form and 2 CFU of the yeast form of B. dermatitidis. No cross-reactivity was observed against other fungal DNA. The assay allowed rapid (5-h) identification of B. dermatitidis from culture and from clinical specimens.
机译:TaqMan实时PCR分析是由 Blastomyces dermatitidis BAD1 基因启动子开发的。该测定法鉴定了所有的皮肤双歧杆菌单倍型和六个阳性石蜡包埋组织中的五个。测定灵敏度的阈值为霉菌双歧杆菌的霉菌形式的1 pg基因组DNA和酵母形式的2 CFU。没有观察到与其他真菌DNA的交叉反应。该测定法允许从培养物和临床标本中快速鉴定(5小时)皮肤病双歧杆菌。

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