Impact of Contaminating DNA in Whole-Genome Amplification Kits Used for Metagenomic Shotgun Sequencing for Infection Diagnosis

摘要

Metagenomic shotgun sequencing is a rapidly developing powerful tool to examine microbial communities. It is also gaining interest in the field of clinical microbiology as a way to identify pathogens in normally sterile specimens (1–7). Not only does this approach allow for pathogen identification, but the gene content information can also be used for other analyses, such as antibiotic resistance prediction (8, 9), typing for tracking of outbreaks or epidemiological studies (10), or assessment for other relevant genes, such as those encoding virulence factors (11, 12).