Species Identification and Strain Typing of Staphylococcus agnetis and Staphylococcus hyicus Isolates from Bovine Milk by Use of a Novel Multiplex PCR Assay and Pulsed-Field Gel Electrophoresis

摘要

Staphylococci are the most common bacteria isolated from the cow's mammary gland and are frequently associated with subclinical or mild clinical mastitis (1–3). Staphylococci can be differentiated into groups using the coagulase test. Hemolytic coagulase-positive isolates are often presumptively identified as Staphylococcus aureus, a so-called “major” contagious mastitis pathogen, and coagulase-negative staphylococci are frequently grouped together as “minor” pathogens (4).