Use of Microsatellite Markers and Gene Dosage To Quantify Gene Copy Numbers in Candida albicans

摘要

With microsatellite marker typing, the number of alleles must be known for calculation of allelic frequencies in the diploid Candida albicans for a given locus. We describe a gene dosage with a double real-time PCR. Such a dosage should also be useful in exploring the loss of heterozygosity in C. albicans.