Evaluation of Five Commercial Real-Time PCR Assays for Detection of Mycoplasma pneumoniae in Respiratory Tract Specimens

摘要

The performances of five commercial TaqMan real-time PCR assays for the detection of Mycoplasma pneumoniae in respiratory tract specimens were evaluated in comparison with an in-house real-time PCR. All kits allowed prompt and specific results, validated by the use of an internal control. The Nanogen kit showed the best clinical sensitivity.