Pyrosequencing as a Rapid Tool for Identification of GES-Type Extended-Spectrum β-Lactamases

摘要

A pyrosequencing technique was used for identification of extended-spectrum β-lactamases (ESBLs) of GES type. These β-lactamases are isolated increasingly emerging in gram-negative bacteria worldwide. This rapid and reliable identification method is interesting, since GES variants, including not only expanded-spectrum cephalosporins but also carbapenems, cephamycins, and monobactams, are the only ESBLs that possess different hydrolysis profiles.