Although substantial epidemiologic evidence links Streptococcusmutans to caries, the pathobiology of caries may involve more complex communities of bacterial species. Molecular methods for bacterial identification and enumeration now make it possible to more precisely study the microbiota associated with dental caries. The purpose of this study was to compare the bacteria found in early childhood caries (ECC) to those found in caries-free children by using molecular identification methods. Cloning and sequencing of bacterial 16S ribosomal DNAs from a healthy subject and a subject with ECC were used for identification of novel species or uncultivated phylotypes and species not previously associated with dental caries. Ten novel phylotypes were identified. A number of species or phylotypes that may play a role in health or disease were identified and warrant further investigation. In addition, quantitative measurements for 23 previously known bacterial species or species groups were obtained by a reverse capture checkerboard assay for 30 subjects with caries and 30 healthy controls. Significant differences were observed for nine species: S. sanguinis was associated with health and, in order of decreasing cell numbers, Actinomycesgerencseriae, Bifidobacterium, S. mutans, Veillonella, S. salivarius, S. constellatus, S. parasanguinis, and Lactobacillusfermentum were associated with caries. These data suggest that A. gerencseriae and other Actinomyces species may play an important role in caries initiation and that a novel Bifidobacterium may be a major pathogen in deep caries. Further investigation could lead to the identification of targets for biological interventions in the caries process and thereby contribute to improved prevention of and treatment for this significant public health problem.
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机译:尽管大量的流行病学证据表明链球菌 em> 变异体 em>与龋齿有关,但龋齿的病理生物学可能涉及更复杂的细菌物种群落。现在,用于细菌鉴定和计数的分子方法使得更精确地研究与龋齿有关的微生物群成为可能。这项研究的目的是通过分子鉴定方法,将儿童早期龋病(ECC)中发现的细菌与无龋儿童中发现的细菌进行比较。来自健康受试者和患有ECC的受试者的细菌16S核糖体DNA的克隆和测序被用于鉴定新物种或未培养的系统型以及先前与龋齿无关的物种。确定了十种新的系统型。鉴定了可能在健康或疾病中起作用的许多物种或系统型,需要进一步调查。另外,通过反向捕获棋盘测定法对30名患有龋齿的受试者和30名健康对照进行了23种先前已知的细菌物种或物种组的定量测量。观察到九种: S em>的显着差异。 sanguinis em>与健康有关,按照细胞数量递减的顺序,放线菌 em> gerencseriae em>,双歧杆菌 em>, S em>。 mutans em>, Veillonella em>, S em>。 salivarius em>, S em>。 constellatus em>, S em>。 parasanguinis em>和乳酸杆菌 em> 发酵菌 em>与龋齿相关。这些数据表明 A em>。 gerencseriae em>和其他放线菌 em>可能在龋齿萌发中起重要作用,而新型 Bifidobacterium em>可能是龋齿的主要病原体。进一步的调查可以确定龋齿过程中生物干预的目标,从而有助于改善对这一重大公共卫生问题的预防和治疗。
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