Comparison of Automated Ribotyping to Pulsed-Field Gel Electrophoresis for Genetic Fingerprinting ofStreptococcus pneumoniae

摘要

Fifty-two isolates of Streptococcus pneumoniaewere characterized by pulsed-field gel electrophoresis (PFGE) and automated ribotyping by using HindIII andPvuII. HindIII ribotypes correlated well with PFGE. PvuII produced fewer bands and was less discriminatory. Automated ribotyping with HindIII is an accurate method for genetic fingerprinting of S. pneumoniae and can complement PFGE.