Application of an Optimized and Highly Discriminatory Method Based on Arbitrarily Primed PCR for Epidemiologic Analysis of Methicillin-Resistant Staphylococcus aureusNosocomial Infections

摘要

The optimization of an arbitrarily primed PCR method for typing 96 methicillin-resistant Staphylococcus aureus (MRSA) isolates was compared with pulsed-field gel electrophoresis. Identical results in the differentiation of MRSA clones and identification of the main cluster that included 82 strains (88% of patients) were obtained by both techniques.