Molecular Typing of a Suspected Cluster of Nocardia farcinica Infections by Use of Randomly Amplified Polymorphic DNA, Pulsed-Field Gel Electrophoresis, and Amplified Fragment Length Polymorphism Analyses

J. S. Kalpoe; K. E. Templeton; A. M. Horrevorts; H. P. Endtz; E. J. Kuijper; A. T. Bernards; C. H. W. Klaassen

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Molecular Typing of a Suspected Cluster of Nocardia farcinica Infections by Use of Randomly Amplified Polymorphic DNA, Pulsed-Field Gel Electrophoresis, and Amplified Fragment Length Polymorphism Analyses

机译：使用随机扩增的多态性DNA，脉冲场凝胶电泳和扩增的片段长度多态性分析对诺卡氏菌诺卡氏菌感染的怀疑分子进行分子分型

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Randomly amplified polymorphic DNA (RAPD), pulsed-field gelelectrophoresis (PFGE), and amplified fragment length polymorphism (AFLP) analyses were used to investigate a possible outbreak of Nocardia farcinica. RAPD and PFGE analyses yielded irreproducible and unsatisfactory results, respectively. AFLP analysis seem to be a promising and welcome addition for molecular analysis of Nocardia isolates.

机译：随机扩增多态性DNA（RAPD），脉冲场凝胶电泳（PFGE）和扩增片段长度多态性（AFLP）分析用于研究 Nocardia farcinica 的可能爆发。 RAPD和PFGE分析分别产生了不可再现和不令人满意的结果。 AFLP分析似乎是对 Nocardia 分离株进行分子分析的有前途和受欢迎的补充。 展开▼

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《Journal of Clinical Microbiology》 |2007年第12期|共3页

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J. S. Kalpoe; K. E. Templeton; A. M. Horrevorts; H. P. Endtz; E. J. Kuijper; A. T. Bernards; C. H. W. Klaassen;
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中图分类医学微生物学（病原细菌学、病原微生物学）;

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专利

1. High-resolution genotyping of Listeria monocytogenes by fluorescent amplified fragment length polymorphism analysis compared to pulsed-field gel electrophoresis, random amplified polymorphic DNA analysis, ribotyping, and PCR-restriction fragment leng [J] . Fonnesbech Vogel B, Fussing V, Ojeniyi B, Journal of food protection . 2004,第8期

机译：通过荧光扩增的片段长度多态性分析与脉冲场凝胶电泳，随机扩增的多态性DNA分析，核糖分型和PCR限制性片段长度比较，对单核细胞增生李斯特菌进行高分辨率基因分型

2. Investigation of a pseudo-outbreak of Nocardia asteroides infection by pulsed-field gel electrophoresis and randomly amplified polymorphic DNA PCR. [J] . L Louie, M Louie, A E Simor Journal of Clinical Microbiology . 1997,第6期

机译：通过脉冲场凝胶电泳和随机扩增的多态性DNA PCR研究假性诺卡氏小行星感染的爆发。

3. Comparative typing of Pseudomonas aeruginosa by random amplification of polymorphic DNA or pulsed-field gel electrophoresis of DNA macrorestriction fragments. [J] . N Renders, Y R?mling, H Verbrugh, Journal of Clinical Microbiology . 1996,第12期

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4. COMPARISON OF AN AUTOMATED RIBOTYPING SYSTEM, PULSED-FIELD GEL ELECTROPHORESIS AND RANDOMLY AMPLIFIED POLYMORPHIC DNA FINGERPRINTING FOR GENOTYPING STREPTOCOCCUS UBERIS [C] . B.E. Gillespie, S.P. Oliver Annual Meeting of the National Mastitis Council . 2003

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5. Amplified fragment length polymorphism (AFLP) in genomic DNA analysis of soybean (Glycine max (L.) Merr.). [D] . Ude, George Nkem. 1999

机译：大豆基因组DNA分析中的扩增片段长度多态性（AFLP）（Glycine max（L.）Merr。）。

6. Molecular Typing of a Suspected Cluster of Nocardia farcinica Infections by Use of Randomly Amplified Polymorphic DNA Pulsed-Field Gel Electrophoresis and Amplified Fragment Length Polymorphism Analyses [O] . J. S. Kalpoe, K. E. Templeton, A. M. Horrevorts, 2007

机译：使用随机扩增的多态性DNA脉冲场凝胶电泳和扩增的片段长度多态性分析对诺卡氏菌诺卡氏菌感染的疑似簇进行分子分型

7. Molecular Typing of a Suspected Cluster of Nocardia farcinica Infections by Use of Randomly Amplified Polymorphic DNA, Pulsed-Field Gel Electrophoresis, and Amplified Fragment Length Polymorphism Analyses▿ [O] . Kalpoe, J. S., Templeton, K. E., Horrevorts, A. M., 2007

机译：利用随机扩增的多态性DNA，脉冲场凝胶电泳和扩增的片段长度多态性分析法对诺卡氏菌诺卡氏菌感染的疑似簇进行分子分型

8. Population Genetics Study of Anopheles Darlingi (Diptera: Culicidae) from Colombia Based on Random Amplified Polymorphic DNA-Polymerase Chain Reaction and Amplified Fragment Length Polymorphism Markers [R] . Gonzalez, R., Wilkerson, R., Suarez, M. F., 2007

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6. 诺卡氏菌腈水合酶的分子模拟与计算机定点突变研究 [C] . 于慧敏 ,史悦 ,刘杰 . 第一届全国化学工程与生物化工年会 . 2004

7. 诺卡氏菌酰胺酶基因的分子克隆及在大肠杆菌中的表达 [A] . 许幂 . 2006

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2. 红色诺卡氏菌细胞壁骨架在制备抗真菌感染的药物中的用途 [P] . 中国专利： CN1879661B . 2012.06.20

3. Control primer annealing to improve the specificity of annealing in nucleic acid amplification kit.Methods for amplifying a nucleic acid sequence of a DNA or a mixture of nucleic acids.And for selectively amplifying a nucleic acid sequence of a target DNA or a mixture of nucleic acids, and a sequence of the target nucleic acid from a mRNA.Method for detecting the complementarity of DNA to mRNA differentially expressed in two or more samples of nucleic acid, methods for amplifying a fragment of the cDNA and DNA quickly target.And to amplify a population of cDNAs of filament double full-length complementary to mRNAs cDNAs, and filament double fortified with 5 'complementary to mRNAs,Method to amplify rather than a sequence of nucleotu00ecdeo target at the same time, methods for producing a digital printing gDNA DNA, and RNA in a sample of mRNA.Method for identifying segments of homology is conserved in a family of multigene from a sample of mRNA, a method for the mutagenesis in a target [P] . 外国专利： BR0214741A . 2004-11-23

机译：控制引物退火，以提高核酸扩增试剂盒中退火的特异性。扩增DNA或核酸混合物的核酸序列的方法以及选择性扩增靶DNA或核酸混合物的核酸序列的方法用于检测DNA与两个或多个核酸样品中差异表达的mRNA的互补性的方法，扩增cDNA和DNA片段的方法可快速靶向。全长与mRNA cDNA互补的双细丝cDNA的cDNA群体，以及与mRNA互补的5'增强双细丝的cDNA，同时扩增而不是核苷酸靶序列的方法，产生数字印刷gDNA DNA的方法mRNA样品中的RNA和RNA.mRNA样品的多基因家族中用于鉴定同源片段的方法是保守的，这是一种在靶标中诱变的方法

4. Control primer annealing to improve the specificity of annealing in nucleic acid amplification, kit, methods for amplifying nucleic acid sequences of DNA and sequence of target nucleic acid or a mixture of nucleic acids, to detect the complementari Dade of DNA to mRNA differentially expressed in two or more samples of nucleic acid.To amplify a fragment of the cDNA target quickly, to amplify a population of cDNAs of filament double full-length complementary to mRNAs cDNAs, and filament double fortified with 5 'complementary to mRNAs to amplify rather than a sequence of nucleotide al VO at the same time, to produce a digital printing gDNA DNA, and RNA in a sample of mRNA.To identify segments of homology is conserved in a multigene family from a sample of mRNA, to identify a variation of nucleotide in a target nucleic acid, for mutagenesis in a target nucleic acid, and use of the initiator [P] . 外国专利： BRPI0214741B1 . 2015-05-12

机译：控制引物退火以改善核酸扩增中退火的特异性，试剂盒，扩增DNA核酸序列和靶核酸序列或核酸混合物的方法，检测DNA与mRNA互补表达的互补性（两种）或更多核酸样品。要快速扩增cDNA靶标的片段，以扩增与mRNA cDNA互补的长丝两倍全长的cDNA群体，并用与mRNA互补的5'双重修饰的长丝进行扩增而不是序列同时鉴定一个核苷酸的VO片段，以产生一个数字印刷的gDNA DNA和一个mRNA样品中的RNA。从一个mRNA样品中鉴定一个多基因家族中的同源片段是保守的，以鉴定一个核苷酸中一个核苷酸的变异。靶核酸，用于在靶核酸中诱变，以及引发剂的用途

5. Method for screening indivu00ecduos of test to identify those having more probably best features of growth, development, reproduction and skeleton, such as rates of gain.The length of the skeleton or the size of the litter and to identify a genetic marker for growth rate of pig, the length of the skeleton, the size of the litter or infection of boar.Kit for assessing a DNA sample of suu00ecno, initiator to test how the presence of a place sph1 polu00ecmero gene CYP11A1 of suu00ecno, genetic marker associated with the rate of growth.The DNA sequence of the 5 'untranslated region of the gene CYP11A1 of suu00ecno, initiator designed to amplify a place of restriction sph1 polymorphic gene CYP11A1 of suu00ecno and method for screening pig. [P] . 外国专利： BR0010537A . 2002-04-16

机译：筛选测试个体的方法以鉴定那些可能具有生长，发育，繁殖和骨骼的最佳特征的动物，例如增重率。骨骼的长度或凋落物的大小，并鉴定生长的遗传标记猪的比率，骨骼的长度，猫砂的大小或公猪的感染。用于评估su u00ecno DNA样本的试剂盒，用于测试su u00eccmero基因sph1 pol u00ecmero基因CYP11A1如何存在的启动子su u00ecno基因CYP11A1的5'非翻译区的DNA序列，用于扩增su u00ecno限制性sph1多态性基因CYP11A1的位点的启动子和猪的筛选方法。

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Molecular Typing of a Suspected Cluster of Nocardia farcinica Infections by Use of Randomly Amplified Polymorphic DNA, Pulsed-Field Gel Electrophoresis, and Amplified Fragment Length Polymorphism Analyses

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