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首页> 外文期刊>Journal of Clinical Microbiology >Rapid Detection of Herpes Simplex Virus DNA in Genital Ulcers by Real-Time PCR Using SYBR Green I Dye as the Detection Signal
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Rapid Detection of Herpes Simplex Virus DNA in Genital Ulcers by Real-Time PCR Using SYBR Green I Dye as the Detection Signal

机译:使用SYBR Green I染料作为检测信号通过实时PCR快速检测生殖器溃疡中的单纯疱疹病毒DNA

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摘要

We have evaluated a real-time PCR procedure based on the LightCycler technology for rapid detection of herpes simplex virus (HSV) in genital lesions. Two sets of primers, corresponding to the thymidine kinase and DNA polymerase regions, were used for the amplification reactions in separate capillaries containing the SYBR Green I dye as detection signal. In 28 of 118 samples (24%), HSV was isolated by conventional cell culture. All cell culture-positive samples were also positive by real-time PCR. Six additional cell culture-negative samples were positive by PCR with both sets of primers. Total processing time was less than 3 h. Real-time PCR using SYBR Green I as detection signal is a sensitive procedure for the rapid diagnosis of HSV in genital lesions.
机译:我们评估了基于LightCycler技术的实时PCR程序,用于快速检测生殖器病变中的单纯疱疹病毒(HSV)。使用两组分别对应于胸苷激酶和DNA聚合酶区域的引物,在包含SYBR Green I染料作为检测信号的独立毛细管中进行扩增反应。在118个样品中的28个(24%)中,通过常规细胞培养分离出HSV。通过实时PCR,所有细胞培养阳性样品也均为阳性。通过两组引物的PCR,另外六个细胞培养阴性样品均为阳性。总处理时间少于3小时。使用SYBR Green I作为检测信号的实时PCR是快速诊断生殖器病变中HSV的敏感程序。

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