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首页> 外文期刊>Journal of Clinical Microbiology >Analysis by Enzyme-Linked Immunosorbent Assay and PCR of Human Liver Abscess Aspirates from Patients in China forEntamoeba histolytica
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Analysis by Enzyme-Linked Immunosorbent Assay and PCR of Human Liver Abscess Aspirates from Patients in China forEntamoeba histolytica

机译:酶联免疫吸附测定法和PCR法分析中国溶血性阿米巴病人肝脓肿

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摘要

Aspirates of liver abscesses were analyzed for Entamoeba histolytica. PCR detected a gene encoding a 30-kDa protein in all samples but detected the ribosomal DNA gene in only 14 (33.3%) samples. Enzyme-linked immunosorbent assay detected antigen in 41 (97.6%) samples. PCR analysis of a strain-specific antigen (SSG) revealed that abscesses were caused by various strains.
机译:分析肝脓肿抽吸物中的溶组织阿莫巴。 PCR在所有样品中检测到一个编码30kDa蛋白的基因,但仅在14个(33.3%)样品中检测到了核糖体DNA基因。酶联免疫吸附法检测到41个(97.6%)样品中的抗原。对菌株特异性抗原(SSG)的PCR分析表明,脓肿是由多种菌株引起的。

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