Rapid discrimination of Mycobacterium tuberculosis complex strains by ligation-mediated PCR fingerprint analysis.

G Prodhom; C Guilhot; M C Gutierrez; A Varnerot; B Gicquel; V Vincent

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Rapid discrimination of Mycobacterium tuberculosis complex strains by ligation-mediated PCR fingerprint analysis.

机译：通过连接介导的PCR指纹分析快速区分结核分枝杆菌复杂菌株。

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A ligation-mediated PCR (LMPCR) method for the amplification of sequences flanking the IS6110 of the Mycobacterium tuberculosis complex has been developed. The method uses one primer specific for IS6110 and a second specific for a linker ligated to SalI-restricted genomic DNA. LMPCR is a rapid screening method, valuable for the fingerprinting of M. tuberculosis complex strains.

机译：已经开发了一种连接介导的PCR（LMPCR）方法，用于扩增结核分枝杆菌复合物IS6110侧翼的序列。该方法使用一种对IS6110具有特异性的引物，以及对与SalI限制性基因组DNA连接的接头具有特异性的第二种引物。 LMPCR是一种快速筛选方法，对于结核分枝杆菌复杂菌株的指纹图谱很有用。

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《Journal of Clinical Microbiology》 |1997年第12期|共4页
作者
G Prodhom; C Guilhot; M C Gutierrez; A Varnerot; B Gicquel; V Vincent;
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中图分类医学微生物学（病原细菌学、病原微生物学）;
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1. Evaluation of a High-Throughput Repetitive-Sequence-Based PCR System for DNA Fingerprinting of Mycobacterium tuberculosis and Mycobacterium avium Complex Strains [J] . Gerard A. Cangelosi, Robert J. Freeman, Kaeryn N. Lewis, Journal of Clinical Microbiology . 2004,第6期

机译：高通量重复序列PCR体系对结核分枝杆菌和鸟分枝杆菌复杂菌株DNA指纹图谱的评估。
2. Rapid discrimination of Mycobacterium tuberculosis strains by random amplified polymorphic DNA analysis. [J] . C J Linton, H Jalal, J P Leeming, Journal of Clinical Microbiology . 1994,第9期

机译：通过随机扩增多态性DNA分析快速区分结核分枝杆菌菌株。
3. Comparison of Ligation-Mediated PCR Methods in Differentiation of Mycobacterium tuberculosis Strains [J] . Anna Zaczek, Anna Brzostek, Arkadiusz Wojtasik, BioMed research international . 2014,第5期

机译：连接介导的PCR方法在结核分枝杆菌菌株鉴别中的比较
4. Application of a rapid and sensitive combined phage-PCR method for the detection of Mycobacterium avium subspecies paratuberculosis in raw milk [C] . George Botsaris, Maria Liapi, Charalambos Kakogiannis, International Colloquium on Paratuberculosis . 2009

机译：快速敏感组合噬菌体PCR方法在原料牛奶中检测分枝杆菌亚种副植入症的应用
5. High throughput genotyping and fingerprinting of Mycobacterium tuberculosis multi-drug resistant strains [D] . Sultana, Razvan 2012

机译：结核分枝杆菌多药耐药菌株的高通量基因分型和指纹图谱
6. Rapid discrimination of Mycobacterium tuberculosis complex strains by ligation-mediated PCR fingerprint analysis. [O] . G Prodhom, C Guilhot, M C Gutierrez, 1997

机译：通过连接介导的PCR指纹分析快速区分结核分枝杆菌复杂菌株。
7. Evaluation of a High-Throughput Repetitive-Sequence-Based PCR System for DNA Fingerprinting of Mycobacterium tuberculosis and Mycobacterium avium Complex Strains [O] . Cangelosi, Gerard A., Freeman, Robert J., Lewis, Kaeryn N., 2004

机译：高通量重复序列PCR体系对结核分枝杆菌和鸟分枝杆菌复杂菌株DNA指纹图谱的评估。
8. Rapid susceptibility testing of Mycobacterium avium complex and Mycobacterium tuberculosis isolated from AIDS patients [R] . Dhople, Arvind M. 1994

机译：从艾滋病患者分离的鸟分枝杆菌复合体和结核分枝杆菌的快速药敏试验

Rapid discrimination of Mycobacterium tuberculosis complex strains by ligation-mediated PCR fingerprint analysis.

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